Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00309  |
| Cognate effector | EFF01482 |
| Related T6SS (Type) | T6SS00005 (Type i4a) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (240 a.a.); Nucleotide sequence (720 bp) |
| Description | TsiT is the immunity protein by that complementation of a plasmid-borne tsiT gene fully restored survival of the prey following co-incubation with the parental strain (PAO1 retS) |
| Reference | [1] Burkinshaw BJ, Liang X, Wong M, et al. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol. 2018 May;3(5):632-640. doi: 10.1038/s41564-018-0144-4. Epub 2018 Apr 9. PMID: 29632369 [2] Wen H, Liu G, Geng Z, et al. Structure and SAXS studies unveiled a novel inhibition mechanism of the Pseudomonas aeruginosa T6SS TseT-TsiT complex. Int J Biol Macromol. 2021 Oct 1;188:450-459. doi: 10.1016/j.ijbiomac.2021.08.029. Epub 2021 Aug 8. PMID: 34371041 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA3908 |
| Coordinates (Strand) | 4376012..4376731 (+) |
| NCBI ID | 15599103 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HXA5_PSEAE |
| KEGG ID | pae:PA3908 |
| PDB ID | 6JDP, 7DYM |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Imm52 | PF15579.8 | 1.1e-25 | 139..234 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-239 | MTDAKAFRRYIFELYFDPARLLELDDDQHLQRIERFLDALAPLHPVLENWYLCGDSLRDALSHNVTEHRQDLAKALSRDRRTRAVELVLWNGEEDPLKGGLSLDYEASGRAVSSRLQLEDAGSLLQVFDAPASSFVAIFLAVLEIWPETTWGMLAPHAYFVHQRTFPDRRSIGWIGFCPHPLRATDFPAATELVDIPGRGTLLLNGREPMDETRREHFERVGEADIKLMELGYLPPLRG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9985 | - | - |
Protein sequence: 240 a.a. .
>IMU00309 NC_002516:4376012-4376731 [Pseudomonas aeruginosa PAO1]
MTDAKAFRRYIFELYFDPARLLELDDDQHLQRIERFLDALAPLHPVLENWYLCGDSLRDALSHNVTEHRQDLAKALSRDR
RTRAVELVLWNGEEDPLKGGLSLDYEASGRAVSSRLQLEDAGSLLQVFDAPASSFVAIFLAVLEIWPETTWGMLAPHAYF
VHQRTFPDRRSIGWIGFCPHPLRATDFPAATELVDIPGRGTLLLNGREPMDETRREHFERVGEADIKLMELGYLPPLRG*
MTDAKAFRRYIFELYFDPARLLELDDDQHLQRIERFLDALAPLHPVLENWYLCGDSLRDALSHNVTEHRQDLAKALSRDR
RTRAVELVLWNGEEDPLKGGLSLDYEASGRAVSSRLQLEDAGSLLQVFDAPASSFVAIFLAVLEIWPETTWGMLAPHAYF
VHQRTFPDRRSIGWIGFCPHPLRATDFPAATELVDIPGRGTLLLNGREPMDETRREHFERVGEADIKLMELGYLPPLRG*
Nucleotide sequence: 720 bp .
>IMU00309 NC_002516:4376012-4376731 [Pseudomonas aeruginosa PAO1]
ATGACGGACGCCAAGGCTTTCAGGCGCTACATATTCGAGCTGTACTTCGATCCGGCACGGCTCCTCGAACTGGACGACGA
CCAGCACCTGCAACGGATAGAACGCTTCCTCGATGCCCTCGCGCCCCTCCATCCGGTGCTGGAGAACTGGTATCTGTGCG
GCGACTCCCTGCGCGATGCCCTCAGCCACAACGTCACCGAGCACCGCCAGGATCTCGCCAAGGCCCTGTCGCGTGACCGA
CGCACCCGGGCGGTGGAACTGGTGCTATGGAACGGCGAGGAGGATCCGCTCAAGGGCGGGTTGTCGCTGGACTACGAGGC
CAGCGGCAGGGCCGTCTCGTCCAGGCTCCAGTTGGAAGATGCCGGCAGCCTGCTGCAGGTGTTCGACGCACCGGCGTCCT
CCTTCGTCGCGATCTTCCTCGCGGTGCTGGAAATCTGGCCCGAAACGACCTGGGGCATGCTCGCTCCGCATGCGTACTTC
GTACACCAGCGGACCTTCCCGGACCGCCGCAGCATCGGCTGGATCGGCTTCTGCCCGCATCCGCTAAGGGCCACGGACTT
CCCGGCGGCTACGGAGCTGGTCGACATTCCCGGCCGTGGCACCCTGCTGCTGAACGGCCGCGAACCGATGGACGAAACCC
GTCGCGAACATTTCGAGCGCGTCGGCGAAGCGGACATCAAGCTGATGGAACTGGGCTACCTGCCGCCGCTGCGCGGCTGA
ATGACGGACGCCAAGGCTTTCAGGCGCTACATATTCGAGCTGTACTTCGATCCGGCACGGCTCCTCGAACTGGACGACGA
CCAGCACCTGCAACGGATAGAACGCTTCCTCGATGCCCTCGCGCCCCTCCATCCGGTGCTGGAGAACTGGTATCTGTGCG
GCGACTCCCTGCGCGATGCCCTCAGCCACAACGTCACCGAGCACCGCCAGGATCTCGCCAAGGCCCTGTCGCGTGACCGA
CGCACCCGGGCGGTGGAACTGGTGCTATGGAACGGCGAGGAGGATCCGCTCAAGGGCGGGTTGTCGCTGGACTACGAGGC
CAGCGGCAGGGCCGTCTCGTCCAGGCTCCAGTTGGAAGATGCCGGCAGCCTGCTGCAGGTGTTCGACGCACCGGCGTCCT
CCTTCGTCGCGATCTTCCTCGCGGTGCTGGAAATCTGGCCCGAAACGACCTGGGGCATGCTCGCTCCGCATGCGTACTTC
GTACACCAGCGGACCTTCCCGGACCGCCGCAGCATCGGCTGGATCGGCTTCTGCCCGCATCCGCTAAGGGCCACGGACTT
CCCGGCGGCTACGGAGCTGGTCGACATTCCCGGCCGTGGCACCCTGCTGCTGAACGGCCGCGAACCGATGGACGAAACCC
GTCGCGAACATTTCGAGCGCGTCGGCGAAGCGGACATCAAGCTGATGGAACTGGGCTACCTGCCGCCGCTGCGCGGCTGA