Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00046  |
| Cognate effector | EFF01279 |
| Related T6SS (Type) | T6SS00005 (Type i4a) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (294 a.a.); Nucleotide sequence (882 bp) |
| Description | PA5088 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA5088 |
| Coordinates (Strand) | 5726357..5727238 (-) |
| NCBI ID | 15600281 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HU95_PSEAE |
| KEGG ID | pae:PA5088 |
| PDB ID | 5JJO |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-293 | MMRPLCFFLLIFLLSGNPVMAAELRPFICVNEKDHLPSLDPQADAWYREAVALAKPDTLRPWDRIVDLYSKAVERGHWKAMHNLASLYRTGWPGGVEKDTQKALDLYQKMIDLKVPQGFYDMAAMIGNRAGVKNPATDGLTFLDKAASLGNPPALTELGRLYIYVAGQDELGLKYTNCAAGQGYAPANYELAMYYRLVAHNYPKAAGYYLLAASQGNDDAAFFMSGVFDKTSPDVDRMWYAPDEKLHKLYDGIYDQLAADPDLRFPNLIKDHPLPPHPTQGYDADRPDWKPGQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9973 | pos: 21-22. VMA-AE. | MMRPLCFFLLIFLLSGNPVMA |
Protein sequence: 294 a.a. .
>IMU00046 NC_002516:c5727238-5726357 [Pseudomonas aeruginosa PAO1]
MMRPLCFFLLIFLLSGNPVMAAELRPFICVNEKDHLPSLDPQADAWYREAVALAKPDTLRPWDRIVDLYSKAVERGHWKA
MHNLASLYRTGWPGGVEKDTQKALDLYQKMIDLKVPQGFYDMAAMIGNRAGVKNPATDGLTFLDKAASLGNPPALTELGR
LYIYVAGQDELGLKYTNCAAGQGYAPANYELAMYYRLVAHNYPKAAGYYLLAASQGNDDAAFFMSGVFDKTSPDVDRMWY
APDEKLHKLYDGIYDQLAADPDLRFPNLIKDHPLPPHPTQGYDADRPDWKPGQ*
MMRPLCFFLLIFLLSGNPVMAAELRPFICVNEKDHLPSLDPQADAWYREAVALAKPDTLRPWDRIVDLYSKAVERGHWKA
MHNLASLYRTGWPGGVEKDTQKALDLYQKMIDLKVPQGFYDMAAMIGNRAGVKNPATDGLTFLDKAASLGNPPALTELGR
LYIYVAGQDELGLKYTNCAAGQGYAPANYELAMYYRLVAHNYPKAAGYYLLAASQGNDDAAFFMSGVFDKTSPDVDRMWY
APDEKLHKLYDGIYDQLAADPDLRFPNLIKDHPLPPHPTQGYDADRPDWKPGQ*
Nucleotide sequence: 882 bp .
>IMU00046 NC_002516:c5727238-5726357 [Pseudomonas aeruginosa PAO1]
ATGATGCGTCCATTATGTTTTTTTCTCTTGATTTTTTTGTTGTCGGGAAATCCTGTCATGGCGGCTGAACTACGACCTTT
TATATGCGTTAACGAAAAAGACCACTTGCCATCTCTAGATCCTCAGGCTGATGCATGGTATCGGGAGGCAGTAGCTCTCG
CCAAGCCCGATACCCTGCGTCCCTGGGATCGTATCGTCGATCTATACAGTAAGGCAGTTGAGCGTGGTCACTGGAAGGCT
ATGCATAACTTGGCGAGCCTCTATCGTACCGGTTGGCCAGGCGGTGTGGAGAAGGATACTCAGAAAGCTCTAGATCTCTA
CCAGAAGATGATCGATTTGAAGGTTCCTCAAGGTTTCTACGATATGGCGGCAATGATCGGAAATCGTGCTGGCGTCAAGA
ATCCTGCGACTGATGGACTTACCTTTCTCGATAAGGCTGCAAGCTTGGGTAATCCACCAGCATTAACTGAGTTAGGAAGG
CTATATATATATGTTGCTGGTCAGGATGAGTTGGGGCTGAAATATACGAACTGTGCTGCAGGCCAGGGCTATGCTCCTGC
CAATTACGAGTTGGCGATGTACTATCGTTTGGTGGCACATAACTACCCAAAGGCGGCAGGCTATTACCTTCTTGCTGCAA
GCCAAGGAAATGATGATGCTGCCTTCTTCATGTCCGGAGTTTTTGATAAGACTAGTCCCGATGTTGACAGAATGTGGTAC
GCCCCCGATGAGAAGCTGCACAAACTATATGATGGTATTTACGATCAACTTGCCGCTGATCCTGATTTGCGTTTTCCCAA
CTTGATAAAGGACCATCCTCTGCCTCCTCACCCGACCCAGGGCTACGACGCAGATCGGCCCGACTGGAAACCGGGGCAGT
GA
ATGATGCGTCCATTATGTTTTTTTCTCTTGATTTTTTTGTTGTCGGGAAATCCTGTCATGGCGGCTGAACTACGACCTTT
TATATGCGTTAACGAAAAAGACCACTTGCCATCTCTAGATCCTCAGGCTGATGCATGGTATCGGGAGGCAGTAGCTCTCG
CCAAGCCCGATACCCTGCGTCCCTGGGATCGTATCGTCGATCTATACAGTAAGGCAGTTGAGCGTGGTCACTGGAAGGCT
ATGCATAACTTGGCGAGCCTCTATCGTACCGGTTGGCCAGGCGGTGTGGAGAAGGATACTCAGAAAGCTCTAGATCTCTA
CCAGAAGATGATCGATTTGAAGGTTCCTCAAGGTTTCTACGATATGGCGGCAATGATCGGAAATCGTGCTGGCGTCAAGA
ATCCTGCGACTGATGGACTTACCTTTCTCGATAAGGCTGCAAGCTTGGGTAATCCACCAGCATTAACTGAGTTAGGAAGG
CTATATATATATGTTGCTGGTCAGGATGAGTTGGGGCTGAAATATACGAACTGTGCTGCAGGCCAGGGCTATGCTCCTGC
CAATTACGAGTTGGCGATGTACTATCGTTTGGTGGCACATAACTACCCAAAGGCGGCAGGCTATTACCTTCTTGCTGCAA
GCCAAGGAAATGATGATGCTGCCTTCTTCATGTCCGGAGTTTTTGATAAGACTAGTCCCGATGTTGACAGAATGTGGTAC
GCCCCCGATGAGAAGCTGCACAAACTATATGATGGTATTTACGATCAACTTGCCGCTGATCCTGATTTGCGTTTTCCCAA
CTTGATAAAGGACCATCCTCTGCCTCCTCACCCGACCCAGGGCTACGACGCAGATCGGCCCGACTGGAAACCGGGGCAGT
GA