Detailed information of regulator
Summary
| Regulator ID | REG0070  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator; QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00004 (Type i1); T6SS00005 (Type i4a) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (504 a.a.); Nucleotide sequence (1512 bp) |
| Description | sfa2 and sfa3 mutants were constructed and expression of the three lacZ fusions were assayed in the mutant backgrounds and H2-T6SS expression was increased in the sfa2 mutant. Sfa2 overproduction led to the decreased expression of H2-T6SS in the WT, and had no effect in the rpoN background. Hence Sfa2 mediates to the indirect repression of H2-T6SS by RpoN.; The right H3-T6SS operon were overexpressed in the rpoN mutant (4.7-fold increase).; Expression of the left H3-T6SS operon decreased 2.0-fold in the rpoN mutant, and complementation of the mutation restored WT expression levels.; The expression of the left and right H3-T6SS operons was significantly decreased in the rhlI mutant (2.4-fold and 2.7-fold respectively).; The expression of the left and right H3-T6SS operons was significantly decreased in the lasR mutant (2.7-fold and 3.3-fold respectively). |
| Reference |
External database links
| Locus tag (Gene) | pa1663 (sfa2) |
| Coordinates (Strand) | 1813395..1814906 (+) |
| NCBI ID | 15596860 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I362_PSEAE |
| KEGG ID | pae:PA1663 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| PA4462 (rpoN) | ↑ | - |
| T6SS transcript | ↑ | - |
| T6SS transcript | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Sigma54_activat | PF00158.28 | 2.4e-69 | 195..362 |
| HTH_8 | PF02954.21 | 1.9e-13 | 454..493 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-503 | MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLPADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQLGSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYGPRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLGGNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEALELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAARELGLPRRTLIYRMGRLNIHFGDPRT |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.7749 | - | - |
Protein sequence of regulator: 504 a.a. .
>REG0070 NC_002516:1813395-1814906 [Pseudomonas aeruginosa PAO1]
MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLP
ADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQL
GSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYG
PRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLG
GNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEAL
ELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAAREL
GLPRRTLIYRMGRLNIHFGDPRT*
MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLP
ADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQL
GSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYG
PRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLG
GNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEAL
ELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAAREL
GLPRRTLIYRMGRLNIHFGDPRT*
Nucleotide sequence of regulator: 1512 bp .
>REG0070 NC_002516:1813395-1814906 [Pseudomonas aeruginosa PAO1]
ATGTTCAGCCGCGTACCGCAACCCCTGGCCTTCGCCGAAGCCCTGCTCGCTCAGTTCGGCGCGCTGTCGCGGGAAACCGA
CATCACGGCCCTGCTGGGCGGTTTCGCACGCGCAGTCGGCGAACTCAGCGGCTGCGAGCTGAGCCAGTTGTATCTGCTCG
ACCAGACCCATACCGCGCTCACCCTCAACGCCGAATGCCGGGACGGCCTGTTGCAGGCGCTGGAGCCGTCGAGCCTGCCG
GCGGACTACACCGGCGAGCAGTTGCTGCAGTTCGCCCTGTGCCAGAACCGCGTGGTCAGTCTCGTCGACCTGCCGTCGCG
GCTGCACGAGACCGGCTTCCTGCCGGCAGGGGAGCAGCCCTGGCTGTCCCTGCTCTGCGTTCCGCTGGTGAGCCCGGAGA
AAACGGTCGAGGGCATGGTGGTCTGCGCTACCCGGCGGCCATTGGACCTGCAGGGATTCGCCCATCCACTGGGCCAGCTG
GGCTCGTTCGTGCTGGCCCAGCTGCATCTGCTGCAACGCCTCTCGCAACCCTCCGGCGAGCCGCAGGGACCGTCCAGCGC
ACCGGTGGTCAGCGGCTACGGGCTGATCGGCAGGAGCGCGGCGATGCGTGAGACCTGCCGCCTGATCAGCAAGGTCCTGC
ACAACCCCTATACCGTGTTGCTGCAGGGCGAGACCGGCACCGGCAAGGAGGTGGTCGCACGGGCGATCCACGAGTACGGC
CCGCGGCGTGCACAGGCGTTCGTGGTGCAGAACTGCGCGGCGTTTCCGGAAAACCTGCTGGAAAGCGAGCTGTTCGGCTA
CCGCAAGGGCGCCTTTACCGGTGCCGAGCGCGATCGTCCCGGGTTGTTCGATGCCGCGGATGGCGGAACCCTGCTGCTCG
ACGAAATCGGCGACATGCCCCTCTCGCTCCAGGCCAAGCTGTTGCGCGTGTTGCAGGAAGGCGAGATTCGCCCGCTCGGC
GGCAACGATACGCACAAGGTCGATGTACGCATCATCGCCGCTACCCACCGTGACCTCGCCCTGCTGGTACGCGATGGCAA
GTTCCGCGAAGACCTCTACTACCGGCTGGCGCAGTTTCCCATCGAGTTGCCGCCGTTGCGCCAGCGCGAGGGCGACGCCC
TCGAACTGGCCCGGCATTTCGCCGAGCGCGCCTGCGTGTTCCTGCGCCGCGCGCCGCTGGGCTGGTCCGCGGAAGCGCTG
GAACTGCTCGACGGCTATGGCTTTCCCGGCAACGTTCGGGAGCTGAAGGGATTGGTGGAATGCGCGGTACTGCTCTGCGA
AGGCGAGATGCTGCTGGCCGAACACTTTTCCCTGCGCGCGCCCCCGGAAGCGGAGGCGCCGCTCAAGCTGCGCGAGCATC
TGGAGCAGATCGAACGGGACCTGCTGCTCGCCTGCCTGCGCAGGAACGCTGGCAACCAGACCCTCGCCGCGCGCGAACTG
GGTCTGCCGCGACGAACCCTGATCTATCGCATGGGACGCCTGAACATTCATTTCGGCGATCCCCGGACCTGA
ATGTTCAGCCGCGTACCGCAACCCCTGGCCTTCGCCGAAGCCCTGCTCGCTCAGTTCGGCGCGCTGTCGCGGGAAACCGA
CATCACGGCCCTGCTGGGCGGTTTCGCACGCGCAGTCGGCGAACTCAGCGGCTGCGAGCTGAGCCAGTTGTATCTGCTCG
ACCAGACCCATACCGCGCTCACCCTCAACGCCGAATGCCGGGACGGCCTGTTGCAGGCGCTGGAGCCGTCGAGCCTGCCG
GCGGACTACACCGGCGAGCAGTTGCTGCAGTTCGCCCTGTGCCAGAACCGCGTGGTCAGTCTCGTCGACCTGCCGTCGCG
GCTGCACGAGACCGGCTTCCTGCCGGCAGGGGAGCAGCCCTGGCTGTCCCTGCTCTGCGTTCCGCTGGTGAGCCCGGAGA
AAACGGTCGAGGGCATGGTGGTCTGCGCTACCCGGCGGCCATTGGACCTGCAGGGATTCGCCCATCCACTGGGCCAGCTG
GGCTCGTTCGTGCTGGCCCAGCTGCATCTGCTGCAACGCCTCTCGCAACCCTCCGGCGAGCCGCAGGGACCGTCCAGCGC
ACCGGTGGTCAGCGGCTACGGGCTGATCGGCAGGAGCGCGGCGATGCGTGAGACCTGCCGCCTGATCAGCAAGGTCCTGC
ACAACCCCTATACCGTGTTGCTGCAGGGCGAGACCGGCACCGGCAAGGAGGTGGTCGCACGGGCGATCCACGAGTACGGC
CCGCGGCGTGCACAGGCGTTCGTGGTGCAGAACTGCGCGGCGTTTCCGGAAAACCTGCTGGAAAGCGAGCTGTTCGGCTA
CCGCAAGGGCGCCTTTACCGGTGCCGAGCGCGATCGTCCCGGGTTGTTCGATGCCGCGGATGGCGGAACCCTGCTGCTCG
ACGAAATCGGCGACATGCCCCTCTCGCTCCAGGCCAAGCTGTTGCGCGTGTTGCAGGAAGGCGAGATTCGCCCGCTCGGC
GGCAACGATACGCACAAGGTCGATGTACGCATCATCGCCGCTACCCACCGTGACCTCGCCCTGCTGGTACGCGATGGCAA
GTTCCGCGAAGACCTCTACTACCGGCTGGCGCAGTTTCCCATCGAGTTGCCGCCGTTGCGCCAGCGCGAGGGCGACGCCC
TCGAACTGGCCCGGCATTTCGCCGAGCGCGCCTGCGTGTTCCTGCGCCGCGCGCCGCTGGGCTGGTCCGCGGAAGCGCTG
GAACTGCTCGACGGCTATGGCTTTCCCGGCAACGTTCGGGAGCTGAAGGGATTGGTGGAATGCGCGGTACTGCTCTGCGA
AGGCGAGATGCTGCTGGCCGAACACTTTTCCCTGCGCGCGCCCCCGGAAGCGGAGGCGCCGCTCAAGCTGCGCGAGCATC
TGGAGCAGATCGAACGGGACCTGCTGCTCGCCTGCCTGCGCAGGAACGCTGGCAACCAGACCCTCGCCGCGCGCGAACTG
GGTCTGCCGCGACGAACCCTGATCTATCGCATGGGACGCCTGAACATTCATTTCGGCGATCCCCGGACCTGA