Detailed information of regulator


Summary

Regulator ID REG0070 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator; QS regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00004 experimental (Type i1); T6SS00005 experimental (Type i4a)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Description sfa2 and sfa3 mutants were constructed and expression of the three lacZ fusions were assayed in the mutant backgrounds and H2-T6SS expression was increased in the sfa2 mutant. Sfa2 overproduction led to the decreased expression of H2-T6SS in the WT, and had no effect in the rpoN background. Hence Sfa2 mediates to the indirect repression of H2-T6SS by RpoN.; The right H3-T6SS operon were overexpressed in the rpoN mutant (4.7-fold increase).; Expression of the left H3-T6SS operon decreased 2.0-fold in the rpoN mutant, and complementation of the mutation restored WT expression levels.; The expression of the left and right H3-T6SS operons was significantly decreased in the rhlI mutant (2.4-fold and 2.7-fold respectively).; The expression of the left and right H3-T6SS operons was significantly decreased in the lasR mutant (2.7-fold and 3.3-fold respectively).
Reference

External database links

Locus tag (Gene) pa1663 (sfa2)
Coordinates (Strand) 1813395..1814906 (+)
NCBI ID 15596860
RefSeq NC_002516
Uniprot ID Q9I362_PSEAE
KEGG ID pae:PA1663
KEGG pathway (determined by BlastKOALA) -
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
PA4462 (rpoN) -
T6SS transcript -
T6SS transcript -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 2.4e-69 195..362
HTH_8 PF02954.21 1.9e-13 454..493

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-503 MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLPADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQLGSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYGPRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLGGNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEALELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAARELGLPRRTLIYRMGRLNIHFGDPRT



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.7749 - -



  Protein sequence of regulator: 504 a.a.    .

>REG0070 NC_002516:1813395-1814906 [Pseudomonas aeruginosa PAO1]
MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLP
ADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQL
GSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYG
PRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLG
GNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEAL
ELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAAREL
GLPRRTLIYRMGRLNIHFGDPRT*

  Nucleotide sequence of regulator: 1512 bp    .

>REG0070 NC_002516:1813395-1814906 [Pseudomonas aeruginosa PAO1]
ATGTTCAGCCGCGTACCGCAACCCCTGGCCTTCGCCGAAGCCCTGCTCGCTCAGTTCGGCGCGCTGTCGCGGGAAACCGA
CATCACGGCCCTGCTGGGCGGTTTCGCACGCGCAGTCGGCGAACTCAGCGGCTGCGAGCTGAGCCAGTTGTATCTGCTCG
ACCAGACCCATACCGCGCTCACCCTCAACGCCGAATGCCGGGACGGCCTGTTGCAGGCGCTGGAGCCGTCGAGCCTGCCG
GCGGACTACACCGGCGAGCAGTTGCTGCAGTTCGCCCTGTGCCAGAACCGCGTGGTCAGTCTCGTCGACCTGCCGTCGCG
GCTGCACGAGACCGGCTTCCTGCCGGCAGGGGAGCAGCCCTGGCTGTCCCTGCTCTGCGTTCCGCTGGTGAGCCCGGAGA
AAACGGTCGAGGGCATGGTGGTCTGCGCTACCCGGCGGCCATTGGACCTGCAGGGATTCGCCCATCCACTGGGCCAGCTG
GGCTCGTTCGTGCTGGCCCAGCTGCATCTGCTGCAACGCCTCTCGCAACCCTCCGGCGAGCCGCAGGGACCGTCCAGCGC
ACCGGTGGTCAGCGGCTACGGGCTGATCGGCAGGAGCGCGGCGATGCGTGAGACCTGCCGCCTGATCAGCAAGGTCCTGC
ACAACCCCTATACCGTGTTGCTGCAGGGCGAGACCGGCACCGGCAAGGAGGTGGTCGCACGGGCGATCCACGAGTACGGC
CCGCGGCGTGCACAGGCGTTCGTGGTGCAGAACTGCGCGGCGTTTCCGGAAAACCTGCTGGAAAGCGAGCTGTTCGGCTA
CCGCAAGGGCGCCTTTACCGGTGCCGAGCGCGATCGTCCCGGGTTGTTCGATGCCGCGGATGGCGGAACCCTGCTGCTCG
ACGAAATCGGCGACATGCCCCTCTCGCTCCAGGCCAAGCTGTTGCGCGTGTTGCAGGAAGGCGAGATTCGCCCGCTCGGC
GGCAACGATACGCACAAGGTCGATGTACGCATCATCGCCGCTACCCACCGTGACCTCGCCCTGCTGGTACGCGATGGCAA
GTTCCGCGAAGACCTCTACTACCGGCTGGCGCAGTTTCCCATCGAGTTGCCGCCGTTGCGCCAGCGCGAGGGCGACGCCC
TCGAACTGGCCCGGCATTTCGCCGAGCGCGCCTGCGTGTTCCTGCGCCGCGCGCCGCTGGGCTGGTCCGCGGAAGCGCTG
GAACTGCTCGACGGCTATGGCTTTCCCGGCAACGTTCGGGAGCTGAAGGGATTGGTGGAATGCGCGGTACTGCTCTGCGA
AGGCGAGATGCTGCTGGCCGAACACTTTTCCCTGCGCGCGCCCCCGGAAGCGGAGGCGCCGCTCAAGCTGCGCGAGCATC
TGGAGCAGATCGAACGGGACCTGCTGCTCGCCTGCCTGCGCAGGAACGCTGGCAACCAGACCCTCGCCGCGCGCGAACTG
GGTCTGCCGCGACGAACCCTGATCTATCGCATGGGACGCCTGAACATTCATTTCGGCGATCCCCGGACCTGA