Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00007  |
| Type | chaperone |
| Related T6SS (Type) | T6SS00005 (Type i4a) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Related structure protein | PA3904 (PAAR4) |
| Related effector | PA3907 (TseT) |
| Description | TecT |
| Reference | [1] Burkinshaw BJ, Liang X, Wong M, et al. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol. 2018 May;3(5):632-640. doi: 10.1038/s41564-018-0144-4. Epub 2018 Apr 9. PMID: 29632369 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA3905 (tecT) |
| Coordinates (Strand) | 4374330..4374857 (+) |
| NCBI ID | 15599100 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HXA8_PSEAE |
| KEGG ID | pae:PA3905 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF4123 | PF13503.8 | 2e-26 | 17..144 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-175 | VSRLQPALAAPTPDSRLFALADGARYLTLDTRLEKAAGEIRSRWLLAGSELDEIAHAGPVLIEFMDSAPLSSSGEFLGWLRDWDRRSPMASWLWSRASFENLAKHFAGLLFTRMPDGRRALLRYYSPEVRRALEQVMTARQWTQVMAPLERWQVWQPLQGGYLVYDRETERTADA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9718 | - | - |
Protein sequence: 176 a.a.
>ACP00007 NC_002516:4374330-4374857 [Pseudomonas aeruginosa PAO1]
VSRLQPALAAPTPDSRLFALADGARYLTLDTRLEKAAGEIRSRWLLAGSELDEIAHAGPVLIEFMDSAPLSSSGEFLGWL
RDWDRRSPMASWLWSRASFENLAKHFAGLLFTRMPDGRRALLRYYSPEVRRALEQVMTARQWTQVMAPLERWQVWQPLQG
GYLVYDRETERTADA*
VSRLQPALAAPTPDSRLFALADGARYLTLDTRLEKAAGEIRSRWLLAGSELDEIAHAGPVLIEFMDSAPLSSSGEFLGWL
RDWDRRSPMASWLWSRASFENLAKHFAGLLFTRMPDGRRALLRYYSPEVRRALEQVMTARQWTQVMAPLERWQVWQPLQG
GYLVYDRETERTADA*
Nucleotide sequence: 528 bp
>ACP00007 NC_002516:4374330-4374857 [Pseudomonas aeruginosa PAO1]
GTGAGCCGGCTCCAGCCGGCGCTCGCGGCGCCTACGCCGGACAGCCGCCTGTTCGCCCTGGCGGACGGCGCCCGCTACCT
GACCCTGGACACCCGCCTGGAAAAGGCCGCGGGGGAAATCCGCTCGCGCTGGCTGCTGGCCGGGAGCGAGCTGGACGAAA
TCGCCCATGCCGGGCCGGTCCTGATCGAATTCATGGACAGCGCCCCGCTTTCGTCATCGGGCGAGTTCCTCGGCTGGCTG
CGCGACTGGGACCGCCGCTCGCCCATGGCCTCCTGGCTGTGGAGCCGGGCGTCCTTCGAAAACCTCGCGAAACACTTCGC
CGGGCTGCTCTTCACCCGCATGCCCGACGGCCGCCGGGCGCTGTTGCGCTACTACAGCCCGGAAGTGCGCCGGGCGCTGG
AACAGGTCATGACCGCGCGGCAATGGACGCAAGTGATGGCGCCGCTGGAGCGTTGGCAGGTCTGGCAGCCCCTGCAGGGC
GGCTACCTCGTCTATGACCGGGAAACGGAGCGGACCGCCGATGCTTGA
GTGAGCCGGCTCCAGCCGGCGCTCGCGGCGCCTACGCCGGACAGCCGCCTGTTCGCCCTGGCGGACGGCGCCCGCTACCT
GACCCTGGACACCCGCCTGGAAAAGGCCGCGGGGGAAATCCGCTCGCGCTGGCTGCTGGCCGGGAGCGAGCTGGACGAAA
TCGCCCATGCCGGGCCGGTCCTGATCGAATTCATGGACAGCGCCCCGCTTTCGTCATCGGGCGAGTTCCTCGGCTGGCTG
CGCGACTGGGACCGCCGCTCGCCCATGGCCTCCTGGCTGTGGAGCCGGGCGTCCTTCGAAAACCTCGCGAAACACTTCGC
CGGGCTGCTCTTCACCCGCATGCCCGACGGCCGCCGGGCGCTGTTGCGCTACTACAGCCCGGAAGTGCGCCGGGCGCTGG
AACAGGTCATGACCGCGCGGCAATGGACGCAAGTGATGGCGCCGCTGGAGCGTTGGCAGGTCTGGCAGCCCCTGCAGGGC
GGCTACCTCGTCTATGACCGGGAAACGGAGCGGACCGCCGATGCTTGA