Detailed information of regulator
Summary
| Regulator ID | REG0297  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111) |
| Related T6SS | T6SS01112 (Type i1) |
| Strain | Vibrio cholerae strain A1552 |
| Replicon | chromosome |
| Sequence | Protein sequence (390 a.a.); Nucleotide sequence (1170 bp) |
| Description | The level of Hcp was reduced in the △cqsA(CAI-1 autoinducer synthase) and △luxS mutants where the level of the hapR expression was expected to be reduced |
| Reference |
External database links
| Locus tag (Gene) | VCA1552_RS17020 (cqsa) |
| Coordinates (Strand) | 455238..456407 (-) |
| NCBI ID | 446962656 |
| RefSeq | NZ_CP024868 |
| Uniprot ID | CQSA_VIBCH, A0A0X1KZY9_VIBCO, A0A0K9UPP1_VIBCL, A0A0H3Q597_VIBCL, C3LVE0_VIBCM, A0A0H6DZT7_VIBCL |
| KEGG ID | vcm:VCM66_A0482, vch:VCA0523, vcq:EN18_01040, vcx:VAA049_2420 |
| KEGG pathway (determined by BlastKOALA) | map02024; map05111 |
| PDB ID | 2WK7, 2WK8, 2WK9, 2WKA, 3KKI, 3HQT |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Hcp expression and secretion | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Aminotran_1_2 | PF00155.23 | 2.3e-30 | 41..375 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-389 | MNKPQLPDFIQNKIDHYIENYFDINKNGKHLVLGKQASPDDIILQSNDYLALANHPLIKARLAKSLLEEQQSLFMSASFLQNDYDKPMIEKRLAKFTGFDECLLSQSGWNANVGLLQTICQPNTNVYIDFFAHMSLWEGARYANAQAHPFMHNNCDHLRMLIQRHGPGIIVVDSIYSTLGTIAPLAELVNISKEFGCALLVDESHSLGTHGPNGAGLLAELGLTREVHFMTASLAKTFAYRAGAIWCNNEVNRCVPFISYPAIFSSTLLPYEAAGLETTLEIIESADNRRQHLDRMARKLRIGLSQLGLTIRSESQIIGLETGDERNTEKVRDYLESNGVFGSVFCRPATSKNKNIIRLSLNSDVNDEQIAKIIEVCSDAVNYGDFYFR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9967 | - | - |
Protein sequence of regulator: 390 a.a. .
>REG0297 NZ_CP024868:c456407-455238 [Vibrio cholerae]
MNKPQLPDFIQNKIDHYIENYFDINKNGKHLVLGKQASPDDIILQSNDYLALANHPLIKARLAKSLLEEQQSLFMSASFL
QNDYDKPMIEKRLAKFTGFDECLLSQSGWNANVGLLQTICQPNTNVYIDFFAHMSLWEGARYANAQAHPFMHNNCDHLRM
LIQRHGPGIIVVDSIYSTLGTIAPLAELVNISKEFGCALLVDESHSLGTHGPNGAGLLAELGLTREVHFMTASLAKTFAY
RAGAIWCNNEVNRCVPFISYPAIFSSTLLPYEAAGLETTLEIIESADNRRQHLDRMARKLRIGLSQLGLTIRSESQIIGL
ETGDERNTEKVRDYLESNGVFGSVFCRPATSKNKNIIRLSLNSDVNDEQIAKIIEVCSDAVNYGDFYFR*
MNKPQLPDFIQNKIDHYIENYFDINKNGKHLVLGKQASPDDIILQSNDYLALANHPLIKARLAKSLLEEQQSLFMSASFL
QNDYDKPMIEKRLAKFTGFDECLLSQSGWNANVGLLQTICQPNTNVYIDFFAHMSLWEGARYANAQAHPFMHNNCDHLRM
LIQRHGPGIIVVDSIYSTLGTIAPLAELVNISKEFGCALLVDESHSLGTHGPNGAGLLAELGLTREVHFMTASLAKTFAY
RAGAIWCNNEVNRCVPFISYPAIFSSTLLPYEAAGLETTLEIIESADNRRQHLDRMARKLRIGLSQLGLTIRSESQIIGL
ETGDERNTEKVRDYLESNGVFGSVFCRPATSKNKNIIRLSLNSDVNDEQIAKIIEVCSDAVNYGDFYFR*
Nucleotide sequence of regulator: 1170 bp .
>REG0297 NZ_CP024868:c456407-455238 [Vibrio cholerae]
ATGAACAAGCCTCAACTTCCTGATTTTATTCAGAACAAGATAGATCACTATATTGAAAATTATTTTGATATAAACAAAAA
CGGTAAACACCTTGTATTGGGTAAACAAGCCAGCCCTGATGACATTATTTTGCAAAGTAATGATTATCTCGCATTGGCCA
ATCATCCGTTGATCAAAGCTCGTTTGGCGAAGTCATTACTGGAAGAACAACAAAGCTTATTTATGTCAGCCTCATTTCTA
CAAAATGACTATGACAAACCCATGATTGAGAAACGTCTGGCTAAGTTCACAGGCTTTGATGAATGTCTATTATCTCAATC
TGGTTGGAATGCAAACGTCGGTTTATTACAAACCATCTGCCAGCCCAATACGAATGTTTACATTGATTTTTTCGCGCACA
TGTCGTTATGGGAAGGGGCGCGCTACGCCAATGCTCAGGCGCATCCTTTTATGCATAATAACTGTGACCATTTACGTATG
CTGATTCAACGTCATGGTCCTGGGATCATTGTCGTAGACTCGATTTACAGCACTTTAGGTACGATTGCACCGCTAGCGGA
ACTGGTCAATATCAGTAAAGAGTTTGGCTGCGCCTTATTAGTCGATGAATCCCACTCTTTGGGCACACATGGCCCTAATG
GTGCAGGTTTATTGGCAGAATTAGGCCTCACTCGTGAAGTGCATTTTATGACCGCAAGTTTGGCCAAAACCTTTGCTTAT
CGCGCAGGAGCCATTTGGTGTAACAATGAAGTGAATCGCTGCGTTCCTTTTATTAGTTATCCAGCTATTTTTAGTTCTAC
TTTGCTGCCTTATGAAGCGGCAGGATTAGAAACGACTTTAGAGATTATTGAATCTGCGGATAATCGTCGTCAGCATTTAG
ATCGTATGGCAAGAAAATTACGCATAGGATTATCCCAGCTGGGATTAACCATTCGCAGTGAAAGCCAAATTATTGGTCTA
GAAACAGGAGATGAACGAAATACCGAAAAAGTTCGGGATTATTTAGAAAGTAATGGAGTGTTTGGCTCAGTATTCTGCCG
CCCGGCAACTTCAAAGAATAAAAACATTATTCGCTTATCACTCAATAGTGATGTGAACGATGAGCAAATCGCCAAAATAA
TTGAGGTTTGCTCTGATGCGGTCAACTACGGTGATTTTTATTTTCGTTAA
ATGAACAAGCCTCAACTTCCTGATTTTATTCAGAACAAGATAGATCACTATATTGAAAATTATTTTGATATAAACAAAAA
CGGTAAACACCTTGTATTGGGTAAACAAGCCAGCCCTGATGACATTATTTTGCAAAGTAATGATTATCTCGCATTGGCCA
ATCATCCGTTGATCAAAGCTCGTTTGGCGAAGTCATTACTGGAAGAACAACAAAGCTTATTTATGTCAGCCTCATTTCTA
CAAAATGACTATGACAAACCCATGATTGAGAAACGTCTGGCTAAGTTCACAGGCTTTGATGAATGTCTATTATCTCAATC
TGGTTGGAATGCAAACGTCGGTTTATTACAAACCATCTGCCAGCCCAATACGAATGTTTACATTGATTTTTTCGCGCACA
TGTCGTTATGGGAAGGGGCGCGCTACGCCAATGCTCAGGCGCATCCTTTTATGCATAATAACTGTGACCATTTACGTATG
CTGATTCAACGTCATGGTCCTGGGATCATTGTCGTAGACTCGATTTACAGCACTTTAGGTACGATTGCACCGCTAGCGGA
ACTGGTCAATATCAGTAAAGAGTTTGGCTGCGCCTTATTAGTCGATGAATCCCACTCTTTGGGCACACATGGCCCTAATG
GTGCAGGTTTATTGGCAGAATTAGGCCTCACTCGTGAAGTGCATTTTATGACCGCAAGTTTGGCCAAAACCTTTGCTTAT
CGCGCAGGAGCCATTTGGTGTAACAATGAAGTGAATCGCTGCGTTCCTTTTATTAGTTATCCAGCTATTTTTAGTTCTAC
TTTGCTGCCTTATGAAGCGGCAGGATTAGAAACGACTTTAGAGATTATTGAATCTGCGGATAATCGTCGTCAGCATTTAG
ATCGTATGGCAAGAAAATTACGCATAGGATTATCCCAGCTGGGATTAACCATTCGCAGTGAAAGCCAAATTATTGGTCTA
GAAACAGGAGATGAACGAAATACCGAAAAAGTTCGGGATTATTTAGAAAGTAATGGAGTGTTTGGCTCAGTATTCTGCCG
CCCGGCAACTTCAAAGAATAAAAACATTATTCGCTTATCACTCAATAGTGATGTGAACGATGAGCAAATCGCCAAAATAA
TTGAGGTTTGCTCTGATGCGGTCAACTACGGTGATTTTTATTTTCGTTAA