Detailed information of regulator
Summary
| Regulator ID | REG0322  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Metabolic pathways (map01100); Cysteine and methionine metabolism (map00270); Quorum sensing (map02024) |
| Related T6SS | T6SS01170 (Type i1) |
| Strain | Pectobacterium brasiliense strain 1692 |
| Replicon | chromosome |
| Sequence | Protein sequence (217 a.a.); Nucleotide sequence (651 bp) |
| Description | In the Pa1043ΔexpI strain, 23 T6SS genes exhibited a strong transcriptional decrease in prey Pa1043 upon the disruption of expI-expR1. |
| Reference |
External database links
| Locus tag (Gene) | GT391_RS06405 (expI) |
| Coordinates (Strand) | 1436931..1437581 (-) |
| NCBI ID | 497970044 |
| RefSeq | NZ_CP047495 |
| Uniprot ID | UPI0001A4453D |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map01100; map00270; map02024 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| GT391_RS06470 (expR1) | ↓ | 746272689 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Autoind_synth | PF00765.19 | 2.9e-88 | 8..187 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-216 | MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITGTFFPYFKEINIPEGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSKDKGYDGIYTIVSHPMLTILKRSGWGIRVVEQGLSEKEERVYLVFLPVDDENQDALVRRINRSGTFMSNELKQWPLRVPAAIAQA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9899 | - | - |
Protein sequence of regulator: 217 a.a. .
>REG0322 NZ_CP047495:c1437581-1436931 [Pectobacterium brasiliense]
MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITG
TFFPYFKEINIPEGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSKDKGYDGIYTIVSHPMLTILKRSGWGIRV
VEQGLSEKEERVYLVFLPVDDENQDALVRRINRSGTFMSNELKQWPLRVPAAIAQA*
MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITG
TFFPYFKEINIPEGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSKDKGYDGIYTIVSHPMLTILKRSGWGIRV
VEQGLSEKEERVYLVFLPVDDENQDALVRRINRSGTFMSNELKQWPLRVPAAIAQA*
Nucleotide sequence of regulator: 651 bp .
>REG0322 NZ_CP047495:c1437581-1436931 [Pectobacterium brasiliense]
ATGTTAGAGATATTTGATGTAAATCACACCTTGTTGTCAGAAACGAAATCAGAAGAGCTATTTACCCTCAGAAAAGAGAC
GTTTAAAGATCGACTGAATTGGGCCGTGCAATGTACTGATGGAATGGAATTTGATCAGTATGATAATAATAACACGACTT
ATCTTTTTGGCATCAAAGACAACACCGTTATCTGTAGTTTGAGATTTATTGAAACAAAATACCCTAACATGATTACTGGG
ACATTTTTTCCTTACTTCAAGGAGATAAATATCCCTGAAGGGAATTACCTGGAATCTAGTCGGTTCTTTGTCGATAAATC
ACGAGCGAAAGATATTCTCGGCAATGAATACCCAATTAGTTCGATGTTGTTTCTTTCAATGATTAATTACTCAAAGGACA
AAGGCTATGATGGAATATATACGATAGTGAGTCACCCTATGCTGACGATATTAAAACGATCTGGCTGGGGAATTCGCGTG
GTCGAACAAGGTTTGTCAGAAAAGGAAGAAAGAGTCTATTTGGTTTTTCTTCCCGTTGATGATGAGAATCAGGATGCATT
GGTTCGTCGTATTAACCGCAGCGGGACATTTATGAGCAATGAATTGAAGCAATGGCCTCTACGGGTACCTGCCGCTATTG
CACAGGCTTGA
ATGTTAGAGATATTTGATGTAAATCACACCTTGTTGTCAGAAACGAAATCAGAAGAGCTATTTACCCTCAGAAAAGAGAC
GTTTAAAGATCGACTGAATTGGGCCGTGCAATGTACTGATGGAATGGAATTTGATCAGTATGATAATAATAACACGACTT
ATCTTTTTGGCATCAAAGACAACACCGTTATCTGTAGTTTGAGATTTATTGAAACAAAATACCCTAACATGATTACTGGG
ACATTTTTTCCTTACTTCAAGGAGATAAATATCCCTGAAGGGAATTACCTGGAATCTAGTCGGTTCTTTGTCGATAAATC
ACGAGCGAAAGATATTCTCGGCAATGAATACCCAATTAGTTCGATGTTGTTTCTTTCAATGATTAATTACTCAAAGGACA
AAGGCTATGATGGAATATATACGATAGTGAGTCACCCTATGCTGACGATATTAAAACGATCTGGCTGGGGAATTCGCGTG
GTCGAACAAGGTTTGTCAGAAAAGGAAGAAAGAGTCTATTTGGTTTTTCTTCCCGTTGATGATGAGAATCAGGATGCATT
GGTTCGTCGTATTAACCGCAGCGGGACATTTATGAGCAATGAATTGAAGCAATGGCCTCTACGGGTACCTGCCGCTATTG
CACAGGCTTGA