Detailed information of regulator
Summary
| Regulator ID | REG0197  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Metabolic pathways (map01100); Cysteine and methionine metabolism (map00270); Quorum sensing (map02024) |
| Related T6SS | T6SS00256  (Type i1) |
| Strain | Yersinia pseudotuberculosis YPIII |
| Replicon | chromosome |
| Sequence | Protein sequence (215 a.a.); Nucleotide sequence (645 bp) |
| Description | the expression of T6SS1 was upregulated in the ypsIytbI and ypsRytbR double mutants compared to the YPIII parent strain. |
| Reference |
External database links
| Locus tag (Gene) | YPK_RS08350 (ypsI) |
| Coordinates (Strand) | 1838406..1839050 (-) |
| NCBI ID | 501053391 |
| RefSeq | NC_010465 |
| Uniprot ID | Q9XDD0_YERPU |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map01100; map00270; map02024 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS transcripts | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Autoind_synth | PF00765.19 | 1.3e-64 | 9..187 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-214 | MLKVFNVNFDRMSENKLDEIFTLRKITFKDRLDWKVTCIDGKESDQYDDENTNYLLGTIDDTLVCSVRFVEMQYPTMITGPFAPYFRDLDLPIDGFIESSRFFVEKALARDKLGNNGSLSAILFLSMVNYARNCGYKGILTVVSRGMYTILKRSGWGITVINQGESEKNEVIYLLHLSIDSNSQQQLIRKIQRVHNIDTHTLESWPLVVPSMTK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9907 | - | - |
Protein sequence of regulator: 215 a.a. .
>REG0197 NC_010465:c1839050-1838406 [Yersinia pseudotuberculosis YPIII]
MLKVFNVNFDRMSENKLDEIFTLRKITFKDRLDWKVTCIDGKESDQYDDENTNYLLGTIDDTLVCSVRFVEMQYPTMITG
PFAPYFRDLDLPIDGFIESSRFFVEKALARDKLGNNGSLSAILFLSMVNYARNCGYKGILTVVSRGMYTILKRSGWGITV
INQGESEKNEVIYLLHLSIDSNSQQQLIRKIQRVHNIDTHTLESWPLVVPSMTK*
MLKVFNVNFDRMSENKLDEIFTLRKITFKDRLDWKVTCIDGKESDQYDDENTNYLLGTIDDTLVCSVRFVEMQYPTMITG
PFAPYFRDLDLPIDGFIESSRFFVEKALARDKLGNNGSLSAILFLSMVNYARNCGYKGILTVVSRGMYTILKRSGWGITV
INQGESEKNEVIYLLHLSIDSNSQQQLIRKIQRVHNIDTHTLESWPLVVPSMTK*
Nucleotide sequence of regulator: 645 bp .
>REG0197 NC_010465:c1839050-1838406 [Yersinia pseudotuberculosis YPIII]
ATGTTAAAAGTATTTAACGTAAACTTTGACAGAATGTCAGAGAATAAATTAGATGAGATCTTTACACTTAGAAAAATAAC
ATTTAAAGATCGTTTGGACTGGAAAGTAACCTGTATTGATGGCAAGGAAAGTGATCAATATGATGATGAGAATACAAATT
ACTTATTAGGCACTATAGACGATACTCTCGTTTGCAGTGTTCGTTTTGTTGAAATGCAGTATCCAACCATGATTACGGGG
CCTTTTGCTCCTTACTTCCGTGATCTGGATTTACCTATTGATGGATTTATTGAGTCGAGCCGCTTTTTTGTCGAAAAAGC
GTTAGCTCGGGATAAATTAGGTAATAATGGTTCTCTCAGTGCAATACTTTTTCTCTCTATGGTCAATTACGCCAGAAACT
GTGGATACAAAGGTATATTGACTGTTGTTAGCAGAGGTATGTATACAATACTGAAACGCTCTGGGTGGGGTATTACTGTT
ATTAATCAAGGTGAATCGGAGAAAAATGAAGTTATCTATCTTTTACACTTGAGCATTGACAGTAATAGCCAACAGCAGTT
GATCAGGAAAATACAACGAGTGCACAATATTGATACTCATACACTTGAAAGCTGGCCGCTTGTTGTGCCCAGTATGACTA
AATAG
ATGTTAAAAGTATTTAACGTAAACTTTGACAGAATGTCAGAGAATAAATTAGATGAGATCTTTACACTTAGAAAAATAAC
ATTTAAAGATCGTTTGGACTGGAAAGTAACCTGTATTGATGGCAAGGAAAGTGATCAATATGATGATGAGAATACAAATT
ACTTATTAGGCACTATAGACGATACTCTCGTTTGCAGTGTTCGTTTTGTTGAAATGCAGTATCCAACCATGATTACGGGG
CCTTTTGCTCCTTACTTCCGTGATCTGGATTTACCTATTGATGGATTTATTGAGTCGAGCCGCTTTTTTGTCGAAAAAGC
GTTAGCTCGGGATAAATTAGGTAATAATGGTTCTCTCAGTGCAATACTTTTTCTCTCTATGGTCAATTACGCCAGAAACT
GTGGATACAAAGGTATATTGACTGTTGTTAGCAGAGGTATGTATACAATACTGAAACGCTCTGGGTGGGGTATTACTGTT
ATTAATCAAGGTGAATCGGAGAAAAATGAAGTTATCTATCTTTTACACTTGAGCATTGACAGTAATAGCCAACAGCAGTT
GATCAGGAAAATACAACGAGTGCACAATATTGATACTCATACACTTGAAAGCTGGCCGCTTGTTGTGCCCAGTATGACTA
AATAG