Detailed information of TA system TA006322

Experimentally validated

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2678240..2678565
• Replicon: chromosome
• Accession: NC_000964
• Organism: Bacillus subtilis subsp. subtilis str. 168
• T1TAdb ID: TA04737

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: P54373
• Locus tag: BSU26050
• Protein ID: NP_390482.1
• Coordinates: 2678240..2678419 (+)
• Length: 60 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	BSU_misc_RNA_81
Coordinates	2678344..2678565 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSU_26050 (BSU26050)	2678240..2678419	+	180	NP_390482.1	toxic peptide of toxin-antitoxin system; skin element	Toxin
-	2678344..2678565	-	222	-	-	Antitoxin
BSU_26055 (BSU26055)	2678799..2678888	+	90	YP_009513980.1	skin region; type I toxin	-
BSU_26060 (BSU26060)	2679142..2679585	-	444	NP_390483.2	putative tail tube protein; skin element	-
BSU_26075 (BSU26075)	2679588..2680988	-	1401	NP_390485.2	putative phage tail sheath protein; skin element	-
BSU_26089 (BSU26089)	2680989..2681180	-	192	YP_003097763.1	conserved phage protein of unknown function; skin element	-
BSU_26090 (BSU26090)	2681177..2681614	-	438	NP_390486.2	conserved phage protein of unknown function; skin element	-
BSU_26100 (BSU26100)	2681627..2682130	-	504	NP_390487.1	putative phage tail component; skin element	-
BSU_26110 (BSU26110)	2682127..2682489	-	363	NP_390488.1	conserved phage protein of unknown function; skin element	-
BSU_26120 (BSU26120)	2682486..2682881	-	396	NP_390489.2	conserved phage protein of unknown function; skin element	-
BSU_26130 (BSU26130)	2682885..2683196	-	312	NP_390490.1	hypothetical protein; skin element	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_022898 Bacillus subtilis PY79 Prophage TA030342 2463344..2517097 0 View NZ_AP012496 Bacillus subtilis BEST7003 Prophage TA030556 2479545..2534896 0.003 View NZ_AP019714 Bacillus subtilis subsp. subtilis strain NBRC 13719 Prophage TA032948 2632843..2694543 0.003 View NZ_AP024621 Bacillus subtilis strain BEST3095 Prophage TA038980 2616843..2678543 0.003 View NZ_AP024622 Bacillus subtilis strain BEST3096 Prophage TA039003 2626920..2678410 0.001 View NZ_AP024623 Bacillus subtilis strain BEST3102 Prophage TA039026 2616352..2667842 0.001 View NZ_AP024624 Bacillus subtilis strain BEST3106 Prophage TA039049 2613567..2665057 0.001 View NZ_AP024625 Bacillus subtilis strain BEST3109 Prophage TA039072 2613847..2665337 0.001 View NZ_AP024626 Bacillus subtilis strain BEST3125 Prophage TA039095 2613585..2665075 0.001 View NZ_AP024627 Bacillus subtilis strain BEST3136 Prophage TA039118 2625206..2675317 0.005 View NZ_AP024628 Bacillus subtilis strain BEST3145 Prophage TA039141 2619529..2674880 0.003 View NZ_CP005997 Bacillus subtilis TO-A Prophage TA045159 2453904..2505394 0.001 View NZ_CP007800 Bacillus subtilis subsp. subtilis str. JH642 substr. AG174 strain JH642 Prophage TA047077 2625759..2675870 0.005 View NZ_CP008698 Bacillus subtilis subsp. subtilis str. AG1839 Prophage TA047271 2625759..2675870 0.005 View NZ_CP010053 Bacillus subtilis strain PS832 Prophage TA050319 2647516..2699006 0.001 View NZ_CP010314 Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet) Prophage TA052009 2624983..2678736 0 View NZ_CP011115 Bacillus subtilis KCTC 1028 = ATCC 6051a strain KCTC 1028 Prophage TA053446 2645490..2699243 0 View NZ_CP011882 Bacillus subtilis strain TO-A JPC Prophage TA055149 3422797..3474287 0.001 View NZ_CP014166 Bacillus subtilis subsp. subtilis strain CU1050 Prophage TA060328 2488438..2543789 0.003 View NZ_CP015004 Bacillus subtilis strain SZMC 6179J isolate B23 Prophage TA062018 2627358..2678848 0.001 View NZ_CP015375 Bacillus subtilis subsp. subtilis strain KCTC 3135 Prophage TA062914 1912895..1974595 0.003 View NZ_CP016852 Bacillus subtilis subsp. subtilis strain 168G Prophage TA065218 2647125..2698615 0.001 View NZ_CP020102 Bacillus subtilis strain NCIB 3610 Prophage TA074094 2647774..2699264 0.001 View NZ_CP032310 Bacillus subtilis strain WB800N Prophage TA111633 2647380..2698870 0.001 View NZ_CP033205 Bacillus subtilis strain MBI 600 Prophage TA113400 2504374..2558127 0 View NZ_CP035167 Bacillus subtilis strain SRCM104008 Prophage TA117290 2485600..2537094 0.01 View NZ_CP035191 Bacillus subtilis strain SRCM104011 Prophage TA117306 2485599..2537092 0.01 View NZ_CP045812 Bacillus subtilis strain P8_B3 Prophage TA142648 2645075..2698828 0 View NZ_CP045821 Bacillus subtilis strain MB8_B7 Prophage TA142701 2628576..2683927 0.003 View NZ_CP045922 Bacillus subtilis strain P8_B1 Prophage TA143021 2647338..2698828 0.001 View NZ_CP047325 Bacillus subtilis strain GOT9 Prophage TA145978 4054160..4116406 0.002 View NZ_CP051860 Bacillus subtilis subsp. subtilis str. 168 Prophage TA155372 2671864..2727215 0.003 View NZ_CP053102 Bacillus subtilis subsp. subtilis str. 168 Prophage TA156136 2766001..2816112 0.005 View NZ_CP058242 Bacillus subtilis strain SP1 Prophage TA166398 2647759..2699249 0.001 View NZ_CP064093 Bacillus subtilis strain N1282-4at Prophage TA180031 2570600..2633747 0.004 View NZ_CP064094 Bacillus subtilis strain N1108-5at Prophage TA180055 2983007..3038358 0.003 View NZ_CP064095 Bacillus subtilis strain N1303-2Ay Prophage TA180078 3475729..3537429 0.003 View NZ_CP065789 Bacillus subtilis strain LBUM979 Prophage TA183391 2647354..2698844 0.001 View NZ_CP075344 Bacillus subtilis subsp. subtilis strain A1 - Midalam Prophage TA203485 2647371..2698858 0.006 View NZ_CP082924 Bacillus subtilis strain FJAT-4 Prophage TA215613 2647343..2698833 0.001 View NZ_CP089592 Bacillus subtilis strain Bsi Prophage TA228408 2642139..2703839 0.003 View NZ_CP093067 Bacillus subtilis strain ATC-3 Prophage TA237655 2046290..2097783 0.009 View NZ_CP093289 Bacillus subtilis strain H2 Prophage TA238025 2547622..2598948 0.009 View NZ_CP094361 Bacillus subtilis strain NCIB 3610 Prophage TA239801 2632760..2694460 0.003 View NZ_CP097130 Bacillus subtilis strain S16 Prophage TA244823 2483287..2537052 0.01 View NZ_CP102769 Bacillus subtilis strain YZSR384 Prophage TA254180 2645084..2698837 0 View NZ_CP103066 Bacillus subtilis strain 0137 Prophage TA254845 2645117..2698870 0 View NZ_CP103783 Bacillus subtilis subsp. subtilis str. 168 Prophage TA256660 2636893..2698593 0.003 View NZ_CP103783 Bacillus subtilis subsp. subtilis str. 168 Prophage TA256660 2644840..2698593 0 View NZ_CP104097 Bacillus subtilis strain GL-4 Prophage TA257161 2672260..2726016 0.008 View NZ_CP104097 Bacillus subtilis strain GL-4 Prophage TA257161 2674522..2726016 0.01 View NZ_CP106673 Bacillus subtilis strain SRCM116268 Prophage TA259624 2493175..2546931 0.008 View NZ_CP112873 Bacillus subtilis strain O4 Prophage TA264916 2647294..2719980 0.008 View NZ_CP112873 Bacillus subtilis strain O4 Prophage TA264916 2649557..2704908 0.003 View NZ_CP113256 Bacillus subtilis strain K1 Prophage TA265662 2491294..2547562 0.01 View NZ_CP115738 Bacillus subtilis strain W7 Prophage TA267368 2653290..2707046 0.008 View NZ_CP120598 Bacillus subtilis strain PRO115 Prophage TA275235 2174939..2236637 0.003 View NZ_CP120613 Bacillus subtilis strain DSM 23521 Prophage TA275251 2448623..2518763 0.006 View NZ_CP120622 Bacillus subtilis strain DSM 1090 Prophage TA275279 2501376..2555129 0 View NZ_CP120681 Bacillus subtilis strain DSM 10 Prophage TA275365 2010216..2063968 0 View NZ_CP125660 Bacillus subtilis strain GUCC48 Prophage TA282266 2645095..2698848 0 View NZ_CP125762 Bacillus subtilis strain KRS015 Prophage TA282346, TA282350, TA282352 1551114..1662348 0.005 View NZ_LN649259 Bacillus subtilis strain BS49 Prophage TA285517 2663499..2717252 0 View NZ_LN680001 Bacillus subtilis strain BS34A Prophage TA285538 2663501..2717254 0 View NZ_LN680001 Bacillus subtilis strain BS34A Prophage TA285538 2665764..2717254 0.001 View NZ_OX419551 Bacillus subtilis isolate NRS6190 Prophage TA296758 2534480..2588233 0 View NZ_OX419563 Bacillus subtilis isolate NRS6153 Prophage TA296813 2606405..2668599 0.004 View NZ_OX419567 Bacillus subtilis isolate NRS6107 Prophage TA296840 2531126..2584879 0 View NZ_OX419569 Bacillus subtilis isolate NRS6118 Prophage TA296861 2682857..2744557 0.003 View NZ_OX419575 Bacillus subtilis isolate NRS6121 Prophage TA296892 2532973..2586726 0 View NZ_OX419580 Bacillus subtilis isolate NRS6116 Prophage TA296945 2694016..2747769 0	inside	Prophage	-	-	2645490..2699243	53753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6739.19 Da
• Isoelectric Point: 8.0370

>T6322 NP_390482.1 NC_000964:2678240-2678419 [Bacillus subtilis subsp. subtilis str. 168]
MSTYESLMVMIGFANLIGGIMTWVISLLTLLFMLRKKDTHPIYITVKEKCLHEDPPIKG

Nucleotide

• Length: 180 bp

>T6322 NC_000964:2678240-2678419 [Bacillus subtilis subsp. subtilis str. 168]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Nucleotide

• Length: 222 bp

>AT6322 NC_000964:c2678565-2678344 [Bacillus subtilis subsp. subtilis str. 168]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10204	Enterococcus faecalis V583	31.579	100	0.353

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P54373

Antitoxin

References

(1) Sylvain Durand et al. (2012) Type I toxin-antitoxin systems in Bacillus subtilis. RNA Biology 9(12):1491-7. [PubMed:23059907]

(2) Jessica M Silvaggi et al. (2005) Small untranslated RNA antitoxin in Bacillus subtilis. Journal of Bacteriology 187(19):6641-50. [PubMed:16166525]

(3) Sylvain Durand et al. (2012) The essential function of B. subtilis RNase III is to silence foreign toxin genes. PLoS Genetics 8(12):e1003181. [PubMed:23300471]


 experimental literature