Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | sprA1-sprA1AS/- |
| Location | 1889647..1889829 | Replicon | chromosome |
| Accession | NC_009641 | ||
| Organism | Staphylococcus aureus subsp. aureus str. Newman | ||
| T1TAdb ID | TA03808 | ||
Toxin (Protein)
| Gene name | sprA1 | Uniprot ID | - |
| Locus tag | NWMN_RS15745 | Protein ID | WP_001801861.1 |
| Coordinates | 1889647..1889742 (+) | Length | 32 a.a. |
Antitoxin (RNA)
| Gene name | sprA1AS | ||
| Locus tag | - | ||
| Coordinates | 1889770..1889829 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NWMN_RS09480 (NWMN_1690) | 1885307..1885933 | + | 627 | WP_000669046.1 | hypothetical protein | - |
| NWMN_RS09485 (NWMN_1691) | 1885974..1886318 | + | 345 | WP_000627551.1 | DUF3969 family protein | - |
| NWMN_RS09490 (NWMN_1692) | 1886416..1886967 | + | 552 | WP_000414205.1 | hypothetical protein | - |
| NWMN_RS09495 (NWMN_1693) | 1887185..1887826 | - | 642 | WP_000494956.1 | ImmA/IrrE family metallo-endopeptidase | - |
| NWMN_RS09500 | 1887940..1888125 | - | 186 | WP_000809857.1 | hypothetical protein | - |
| NWMN_RS09505 | 1888127..1888303 | - | 177 | WP_000375476.1 | hypothetical protein | - |
| NWMN_RS09510 (NWMN_1694) | 1888314..1888697 | - | 384 | WP_000070811.1 | hypothetical protein | - |
| NWMN_RS09520 (NWMN_1695) | 1889301..1889444 | - | 144 | WP_001549059.1 | transposase | - |
| NWMN_RS15745 | 1889647..1889742 | + | 96 | WP_001801861.1 | type I toxin-antitoxin system Fst family toxin PepA1 | Toxin |
| - | 1889770..1889829 | - | 60 | - | - | Antitoxin |
| NWMN_RS09525 | 1889865..1889966 | + | 102 | WP_001791893.1 | hypothetical protein | - |
| NWMN_RS15395 | 1889944..1890120 | - | 177 | Protein_1829 | transposase | - |
| NWMN_RS09530 (NWMN_1696) | 1890314..1890691 | - | 378 | WP_001037045.1 | DUF1433 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Prophage | - | lukD / hlgA | 1882747..1922929 | 40182 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 32 a.a. Molecular weight: 3554.32 Da Isoelectric Point: 10.4449
>T10024 WP_001801861.1 NC_009641:1889647-1889742 [Staphylococcus aureus subsp. aureus str. Newman]
VMLIFVHIIAPVISGCAIAFFSYWLSRRNTK
VMLIFVHIIAPVISGCAIAFFSYWLSRRNTK
Download Length: 96 bp
>T10024 NC_009641:1889647-1889742 [Staphylococcus aureus subsp. aureus str. Newman]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG
Antitoxin
Download Length: 60 bp
>AT10024 NC_009641:c1889829-1889770 [Staphylococcus aureus subsp. aureus str. Newman]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10149 | Staphylococcus aureus subsp. aureus str. Newman |
46.429 |
90.323 |
0.419 |
| T10028 | Enterococcus faecalis V583 |
35 |
86.957 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Nour Sayed et al. (2012) Functional and structural insights of a Staphylococcus aureus apoptotic-like membrane peptide from a toxin-antitoxin module. The Journal of Biological Chemistry 287(52):43454-63. [PubMed:23129767]
(2) Nour Sayed et al. (2011) A cis-antisense RNA acts in trans in Staphylococcus aureus to control translation of a human cytolytic peptide. Nature Structural & Molecular Biology 19(1):105-12. [PubMed:22198463]