Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0005	RT90_16255 (wecG)	T6SS01264	protein	TCS regulatory pathway	Serratia marcescens strain RM66262	chromosome (JWLO01000003)	92056..92796 (-)	735005313	RT90_01105 (rcsC) (↑)
REG0013	Y880_03649 (suhB)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	TCS regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	3616623..3617438 (+)	489183406	Y880_02189 (gacA/uvrY) (↓)
REG0086	exoR	T6SS00016	protein	TCS regulatory pathway	Agrobacterium fabrum str. C58	chromosome (NC_003062)	1702398..1703192 (-)		chvG (↑)
REG0173	FTN_RS06610 (mglA)	T6SS00158	protein	TCS regulatory pathway	Francisella tularensis subsp. novicida U112	chromosome (NC_008601)	1362282..1362899 (+)	489124662	iglC transcription (↓); Unknown (↓)
REG0174	FTN_RS02845 (SspA)	T6SS00158	protein	TCS regulatory pathway	Francisella tularensis subsp. novicida U112	chromosome (NC_008601)	574840..575472 (+)	500053699	FTN_1465 (pmrA) (↓)
REG0176	FTN_1465 (pmrA)	T6SS00158	protein	TCS-RR; TCS regulatory pathway	Francisella tularensis subsp. novicida U112	chromosome (NC_008601)	1553358..1554044 (-)	489108239	FTN_1465 (pmrA) promotor (↓); pdpD promotor (↓); IglA expression (↓)
REG0177	FTN_RS06615 (mglB)	T6SS00158	protein	Global regulator; TCS regulatory pathway	Francisella tularensis subsp. novicida U112	chromosome (NC_008601)	1362901..1363308 (+)	489112095	IglA expression (↓); T6SS Transciption (↓)
REG0178	FTN_0480 (fevR/PigR)	T6SS00158	protein	TCS regulatory pathway	Francisella tularensis subsp. novicida U112	chromosome (NC_008601)	486541..486876 (+)	489108070	T6SS Transciption (↓)
REG0205	NT01EI_RS04200 (EsrC)	T6SS00346	protein	TCS regulatory pathway	Edwardsiella ictaluri 93-146	chromosome (NC_012779)	933326..934018 (-)	737638726	T6SS translocon protein EvpC secretion (↓)
REG0206	ETAE_2873 (RpoS)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3025094..3026080 (-)	502612732	ETAE_0886 (esrB) promoter (↑)
REG0207	ETAE_0035	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	35881..37008 (+)	502609898	ETAE_0886 (esrB) promoter (↑)
REG0208	ETAE_0154 (hemB)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	159867..160889 (+)	502610017	ETAE_0886 (esrB) promoter (↑)
REG0209	ETAE_0743 (cdsA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	820052..820909 (+)	502610607	ETAE_0886 (esrB) promoter (↑)
REG0210	ETAE_2082 (pyrF)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2196136..2196858 (-)	502611934	ETAE_0886 (esrB) promoter (↑)
REG0211	ETAE_1096 (dapA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1194778..1195659 (+)	502610962	ETAE_0886 (esrB) promoter (↑)
REG0212	ETAE_2697 (cysS)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2831230..2832615 (-)	502612557	ETAE_0886 (esrB) promoter (↑)
REG0213	ETAE_1760	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (CP001135)	1870377..1870535 (+)	267984768	ETAE_0886 (esrB) promoter (↑)
REG0214	ETAE_1837	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (CP001135)	1946159..1946284 (-)	267984845	ETAE_0886 (esrB) promoter (↑)
REG0215	ETAE_1202 (ugd)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1306603..1307769 (+)	502611067	ETAE_0886 (esrB) promoter (↑)
REG0216	ETAE_2640 (holA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2779832..2780866 (+)	502612494	ETAE_0886 (esrB) promoter (↑)
REG0217	ETAE_1439 (ruvA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1550776..1551384 (+)	502611302	ETAE_0886 (esrB) promoter (↑)
REG0218	ETAE_0358 (purA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	403538..404836 (+)	502610221	ETAE_0886 (esrB) promoter (↑)
REG0219	ETAE_2813 (iscA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2952011..2952334 (-)	491431780	ETAE_0886 (esrB) promoter (↑)
REG0220	ETAE_2787 (guaB)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2925310..2926776 (-)	502612646	ETAE_0886 (esrB) promoter (↑)
REG0221	ETAE_1412	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1522924..1523409 (-)	502611274	ETAE_0886 (esrB) promoter (↑)
REG0222	ETAE_0430	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	473075..473755 (-)	502610291	ETAE_0886 (esrB) promoter (↑)
REG0223	ETAE_0659 (aceE)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	731608..734271 (+)	502610523	ETAE_0886 (esrB) promoter (↑)
REG0224	ETAE_2773 (mtnN)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2910743..2911441 (+)	502612632	ETAE_0886 (esrB) promoter (↑)
REG0225	ETAE_2468	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2598484..2599356 (-)	504339400	ETAE_0886 (esrB) promoter (↑)
REG0226	ETAE_1614	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1721332..1722024 (-)	754045576	ETAE_0886 (esrB) promoter (↑)
REG0227	ETAE_0994 (Lon)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1085063..1087288 (+)	267984010	ETAE_0886 (esrB) promoter (↓)
REG0228	ETAE_3542 (phoU)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3747537..3748268 (+)	502613380	ETAE_0886 (esrB) promoter (↓)
REG0229	ETAE_3449 (tpiA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3637381..3638145 (+)	502613286	ETAE_0886 (esrB) promoter (↓)
REG0230	ETAE_1655 (prc)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1757898..1759940 (+)	735677771	ETAE_0886 (esrB) promoter (↓)
REG0231	ETAE_1158	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1257412..1258584 (+)	502611022	ETAE_0886 (esrB) promoter (↓)
REG0232	ETAE_0772 (tadA)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	850367..850879 (+)	505698653	ETAE_0886 (esrB) promoter (↓)
REG0233	ETAE_0695 (mrcB)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	766885..769368 (+)	502610560	ETAE_0886 (esrB) promoter (↓)
REG0234	ETAE_1170	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (CP001135)	1271304..1271447 (-)	267984186	ETAE_0886 (esrB) promoter (↓)
REG0235	ETAE_1562	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1668753..1670378 (-)	780104675	ETAE_0886 (esrB) promoter (↓)
REG0236	ETAE_3453 (cpxR)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3641204..3641902 (+)	502613290	ETAE_0886 (esrB) promoter (↓)
REG0237	ETAE_0263 (mltC)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	293215..294306 (-)	1392602598	ETAE_0886 (esrB) promoter (↓)
REG0238	ETAE_0542 (slt)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	588958..590886 (+)	502610404	ETAE_0886 (esrB) promoter (↓)
REG0239	ETAE_2638 (leuS)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	2776570..2779152 (+)	502612492	ETAE_0886 (esrB) promoter (↓)
REG0240	ETAE_3207 (rplQ)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3390207..3390593 (-)	491439763	ETAE_0886 (esrB) promoter (↓)
REG0241	ETAE_1010 (acrB)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1099309..1102461 (-)	502610875	ETAE_0886 (esrB) promoter (↓)
REG0242	ETAE_1425 (mviN)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	1535368..1536906 (+)	502611288	ETAE_0886 (esrB) promoter (↓)
REG0274	W817_01610 (NplE)	T6SS00937	protein	TCS regulatory pathway	Escherichia coli RS218	chromosome (NZ_CP007149)	214947..215657 (+)	446161331	W817_RS21690 (CpxA) (↓)
REG0340	BN49_RS29275 (mgrB)	T6SS01214	protein	TCS regulatory pathway	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	3583038..3583181 (-)	489000713	phoPQ (↑)