SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0013
Regulator type	protein
Regulatory pathway (from literatures)	TCS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Metabolic pathways (map01100); Biosynthesis of secondary metabolites (map01110); Inositol phosphate metabolism (map00562); Streptomycin biosynthesis (map00521); Phosphatidylinositol signaling system (map04070)
Related T6SS	T6SS01211 (Type i1); T6SS01212 (Type i4a); T6SS01213 (Type i3)
Strain	Pseudomonas aeruginosa PAK
Replicon	chromosome
Sequence	Protein sequence (272 a.a.); Nucleotide sequence (816 bp)
Description	SuhB regulates the expression of RsmY/Z through GacA and regulates the expression of Hcp1 through GacA and RsmY/Z, similar to their effect on the T3SS.
Reference	[1] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572

External database links

Locus tag (Gene)	Y880_03649 (suhB)
Coordinates (Strand)	3616623..3617438 (+)
NCBI ID	489183406
RefSeq	NZ_CP020659
Uniprot ID	V6A9X5_PSEAI, A0A1C7BM28_PSEAI, A0A1F0IC59_9PSED, A0A0H2ZE36_PSEAB, SUHB_PSEAE
KEGG ID	pae:PA3818, pau:PA14_14680
KEGG pathway (determined by BlastKOALA)	map01100; map01110; map00562; map00521; map04070
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
Y880_02189 (gacA/uvrY)	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Inositol_P	PF00459.27	1.3e-65	1..258

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-271	MQPMLNIALRAARSAGELIFRSIERLDVISVNEKDAKDYVTEVDRAAEQTIVAALRKAYPTHAIMGEEGGFIEGSGEGADYLWVIDPLDGTTNFIHGVPHFAVSIACKYKGRLEHAVVLDPVRQEEFTASRGRGAALNGRRLRVSGRKSLEGALLGTGFPFRDNQIDNLDNYLNMFRSLVGQTAGIRRAGAASLDLAYVAAGRYDAFWEFGLSEWDMAAGALLVQEAGGLVSDFTGSHEFLEKGHIVAGNTKCFKALLTTIQPHLPPSLKR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9683	-	-

Protein sequence of regulator: 272 a.a. .

                                    >REG0013 NZ_CP020659:3616623-3617438 [Pseudomonas aeruginosa PAK]
MQPMLNIALRAARSAGELIFRSIERLDVISVNEKDAKDYVTEVDRAAEQTIVAALRKAYPTHAIMGEEGGFIEGSGEGAD
YLWVIDPLDGTTNFIHGVPHFAVSIACKYKGRLEHAVVLDPVRQEEFTASRGRGAALNGRRLRVSGRKSLEGALLGTGFP
FRDNQIDNLDNYLNMFRSLVGQTAGIRRAGAASLDLAYVAAGRYDAFWEFGLSEWDMAAGALLVQEAGGLVSDFTGSHEF
LEKGHIVAGNTKCFKALLTTIQPHLPPSLKR*

                                 

Nucleotide sequence of regulator: 816 bp .

                                    >REG0013 NZ_CP020659:3616623-3617438 [Pseudomonas aeruginosa PAK]
ATGCAGCCTATGCTGAATATCGCCCTGCGCGCCGCTCGCAGCGCCGGTGAACTGATTTTCCGCTCCATCGAACGCCTGGA
TGTCATCTCGGTCAACGAGAAGGATGCCAAGGACTACGTGACCGAAGTCGATCGCGCCGCCGAGCAGACCATCGTCGCCG
CGCTGCGCAAGGCCTATCCGACTCACGCGATCATGGGCGAGGAAGGCGGCTTCATCGAAGGCAGCGGCGAGGGTGCCGAT
TACCTCTGGGTGATCGATCCGCTGGACGGCACCACCAACTTCATCCATGGCGTACCGCACTTCGCCGTGAGCATCGCCTG
CAAGTACAAAGGCCGCCTGGAGCACGCCGTGGTCCTCGACCCGGTACGCCAGGAAGAATTCACCGCCAGCCGCGGTCGCG
GCGCCGCCCTCAACGGTCGCCGCCTGCGCGTCAGCGGCCGCAAGAGCCTGGAAGGCGCCCTGCTCGGCACCGGGTTCCCG
TTCCGCGACAACCAGATCGACAATCTCGACAACTACCTGAACATGTTCCGCAGCCTGGTCGGCCAGACCGCCGGCATCCG
CCGCGCCGGCGCCGCCAGCCTGGACCTGGCCTACGTCGCCGCCGGACGCTACGACGCCTTCTGGGAATTCGGCCTGTCCG
AATGGGACATGGCGGCAGGCGCCCTGCTGGTACAGGAAGCCGGCGGCCTGGTGAGCGACTTCACCGGCAGCCACGAATTC
CTCGAGAAGGGTCACATCGTCGCCGGCAACACCAAATGCTTCAAGGCACTGCTGACCACCATCCAGCCGCACCTGCCGCC
GTCGCTGAAGCGCTGA

                                