20321

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2324200..2324671 Replicon chromosome
Accession NC_002655
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag Z_RS12145 Protein ID WP_001303511.1
Coordinates 2324393..2324671 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag Z_RS12140 Protein ID WP_001302048.1
Coordinates 2324200..2324391 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Z_RS12095 (Z6065) 2319407..2319991 - 585 WP_000206793.1 DUF551 domain-containing protein -
Z_RS12100 (Z6066) 2320048..2320443 - 396 WP_001118159.1 DUF977 family protein -
Z_RS12105 (Z6067) 2320459..2321228 - 770 Protein_2314 DUF1627 domain-containing protein -
Z_RS12115 (Z6069) 2321254..2321994 - 741 WP_000788938.1 ATP-binding protein -
Z_RS12120 (Z6070) 2322001..2322963 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
Z_RS12125 (Z6071) 2322986..2323411 - 426 WP_000693943.1 Rha family transcriptional regulator -
Z_RS12130 (Z6072) 2323408..2323710 - 303 WP_000172738.1 transcriptional regulator -
Z_RS30220 (Z6073) 2323808..2324179 + 372 WP_001169686.1 hypothetical protein -
Z_RS12140 (Z6074) 2324200..2324391 + 192 WP_001302048.1 hypothetical protein Antitoxin
Z_RS12145 2324393..2324671 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Z_RS12155 (Z6075) 2325024..2325323 + 300 WP_001240334.1 hypothetical protein -
Z_RS12160 (Z6076) 2325395..2325613 + 219 WP_001171930.1 hypothetical protein -
Z_RS30230 2326182..2326370 + 189 WP_000449192.1 cell division inhibition protein DicB -
Z_RS12165 (Z6079) 2326367..2326558 + 192 WP_001090200.1 DUF1482 family protein -
Z_RS12170 (Z6080) 2326651..2329122 + 2472 WP_000048458.1 exonuclease -
Z_RS12175 (Z6081) 2329195..2329446 + 252 WP_000005552.1 excisionase family protein -
Z_RS32390 (Z2566) 2329481..2329665 + 185 Protein_2328 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2282958..2329636 46678


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T20321 WP_001303511.1 NC_002655:2324393-2324671 [Escherichia coli O157:H7 str. EDL933]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T20321 NC_002655:2324393-2324671 [Escherichia coli O157:H7 str. EDL933]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT20321 WP_001302048.1 NC_002655:2324200-2324391 [Escherichia coli O157:H7 str. EDL933]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT20321 NC_002655:2324200-2324391 [Escherichia coli O157:H7 str. EDL933]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References