Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3149478..3149949 Replicon chromosome
Accession NZ_CP062725
Organism Escherichia coli O157:H7 strain Z1813

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ52_RS16150 Protein ID WP_001303511.1
Coordinates 3149671..3149949 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ52_RS16145 Protein ID WP_001302048.1
Coordinates 3149478..3149669 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ52_RS16105 3144685..3145269 - 585 WP_000206793.1 DUF551 domain-containing protein -
ILQ52_RS16110 3145326..3145721 - 396 WP_001118159.1 DUF977 family protein -
ILQ52_RS16115 3145737..3146506 - 770 Protein_3153 DUF1627 domain-containing protein -
ILQ52_RS16120 3146532..3147272 - 741 WP_000788938.1 ATP-binding protein -
ILQ52_RS16125 3147279..3148241 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ52_RS16130 3148264..3148689 - 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ52_RS16135 3148686..3148988 - 303 WP_000172738.1 transcriptional regulator -
ILQ52_RS16140 3149086..3149457 + 372 WP_001169686.1 hypothetical protein -
ILQ52_RS16145 3149478..3149669 + 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ52_RS16150 3149671..3149949 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ52_RS16155 3150302..3150601 + 300 WP_001240334.1 hypothetical protein -
ILQ52_RS16160 3150673..3150891 + 219 WP_001171930.1 hypothetical protein -
ILQ52_RS16165 3151460..3151648 + 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ52_RS16170 3151645..3151836 + 192 WP_001090200.1 DUF1482 family protein -
ILQ52_RS16175 3151929..3154400 + 2472 WP_072612735.1 exonuclease -
ILQ52_RS16180 3154465..3154713 + 249 WP_000113189.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 3108414..3157605 49191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T175780 WP_001303511.1 NZ_CP062725:3149671-3149949 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T175780 NZ_CP062725:3149671-3149949 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT175780 WP_001302048.1 NZ_CP062725:3149478-3149669 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT175780 NZ_CP062725:3149478-3149669 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References