Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2606845..2607316 Replicon chromosome
Accession NZ_CP038346
Organism Escherichia coli O157:H7 strain G5295

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E4U77_RS13125 Protein ID WP_001303511.1
Coordinates 2606845..2607123 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E4U77_RS13130 Protein ID WP_001302048.1
Coordinates 2607125..2607316 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U77_RS13085 2601851..2602035 - 185 Protein_2555 DUF3596 domain-containing protein -
E4U77_RS13090 2602070..2602321 - 252 WP_000005552.1 excisionase family protein -
E4U77_RS13095 2602394..2604865 - 2472 WP_000048458.1 exonuclease -
E4U77_RS13100 2604958..2605149 - 192 WP_001090200.1 DUF1482 family protein -
E4U77_RS13105 2605146..2605334 - 189 WP_000449192.1 cell division inhibition protein DicB -
E4U77_RS13110 2605735..2605899 + 165 WP_001331716.1 hypothetical protein -
E4U77_RS13115 2605903..2606121 - 219 WP_001171930.1 hypothetical protein -
E4U77_RS13120 2606193..2606492 - 300 WP_001240334.1 hypothetical protein -
E4U77_RS13125 2606845..2607123 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U77_RS13130 2607125..2607316 - 192 WP_001302048.1 hypothetical protein Antitoxin
E4U77_RS13135 2607337..2607708 - 372 WP_001169686.1 hypothetical protein -
E4U77_RS13140 2607806..2608108 + 303 WP_000172738.1 transcriptional regulator -
E4U77_RS13145 2608105..2608530 + 426 WP_000693943.1 Rha family transcriptional regulator -
E4U77_RS13150 2608553..2609515 + 963 Protein_2568 helix-turn-helix domain-containing protein -
E4U77_RS13155 2609522..2610262 + 741 WP_000788938.1 ATP-binding protein -
E4U77_RS13160 2610288..2611057 + 770 Protein_2570 DUF1627 domain-containing protein -
E4U77_RS13165 2611073..2611468 + 396 WP_001118159.1 DUF977 family protein -
E4U77_RS13170 2611525..2612109 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2598558..2648554 49996


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121740 WP_001303511.1 NZ_CP038346:c2607123-2606845 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121740 NZ_CP038346:c2607123-2606845 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121740 WP_001302048.1 NZ_CP038346:c2607316-2607125 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121740 NZ_CP038346:c2607316-2607125 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References