Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2398216..2398687 Replicon chromosome
Accession NZ_CP016755
Organism Escherichia coli strain FORC_044

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag FORC44_RS12745 Protein ID WP_001303511.1
Coordinates 2398216..2398494 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag FORC44_RS12750 Protein ID WP_001302048.1
Coordinates 2398496..2398687 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC44_RS12705 2393441..2393692 - 252 WP_000005552.1 excisionase family protein -
FORC44_RS12710 2393765..2396236 - 2472 WP_000048458.1 exonuclease -
FORC44_RS12715 2396329..2396520 - 192 WP_001090200.1 DUF1482 family protein -
FORC44_RS12720 2396517..2396705 - 189 WP_000449192.1 cell division inhibition protein DicB -
FORC44_RS12730 2397274..2397492 - 219 WP_001171930.1 hypothetical protein -
FORC44_RS12735 2397564..2397863 - 300 WP_001240334.1 hypothetical protein -
FORC44_RS12745 2398216..2398494 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FORC44_RS12750 2398496..2398687 - 192 WP_001302048.1 hypothetical protein Antitoxin
FORC44_RS12755 2398708..2399079 - 372 WP_001169686.1 hypothetical protein -
FORC44_RS12760 2399177..2399479 + 303 WP_000172738.1 transcriptional regulator -
FORC44_RS12765 2399476..2399901 + 426 WP_000693943.1 Rha family transcriptional regulator -
FORC44_RS12770 2399924..2400886 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
FORC44_RS12775 2400893..2401633 + 741 WP_000788938.1 ATP-binding protein -
FORC44_RS12780 2401659..2402428 + 770 Protein_2332 DUF1627 domain-containing protein -
FORC44_RS12785 2402444..2402839 + 396 WP_001118159.1 DUF977 family protein -
FORC44_RS12790 2402896..2403480 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2391055..2435644 44589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T65004 WP_001303511.1 NZ_CP016755:c2398494-2398216 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T65004 NZ_CP016755:c2398494-2398216 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT65004 WP_001302048.1 NZ_CP016755:c2398687-2398496 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT65004 NZ_CP016755:c2398687-2398496 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References