Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2556988..2557459 Replicon chromosome
Accession NZ_CP066753
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag JG531_RS12880 Protein ID WP_001303511.1
Coordinates 2556988..2557266 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag JG531_RS12885 Protein ID WP_001302048.1
Coordinates 2557268..2557459 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS12845 2551994..2552178 - 185 Protein_2507 DUF3596 domain-containing protein -
JG531_RS12850 2552213..2552464 - 252 WP_000005552.1 excisionase family protein -
JG531_RS12855 2552537..2555008 - 2472 WP_000048458.1 exonuclease -
JG531_RS12860 2555101..2555292 - 192 WP_001090200.1 DUF1482 family protein -
JG531_RS12865 2555289..2555477 - 189 WP_000449192.1 cell division inhibition protein DicB -
JG531_RS12870 2556046..2556264 - 219 WP_001171930.1 hypothetical protein -
JG531_RS12875 2556336..2556635 - 300 WP_001240334.1 hypothetical protein -
JG531_RS12880 2556988..2557266 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JG531_RS12885 2557268..2557459 - 192 WP_001302048.1 hypothetical protein Antitoxin
JG531_RS12890 2557480..2557851 - 372 WP_001169686.1 hypothetical protein -
JG531_RS12895 2557949..2558251 + 303 WP_000172738.1 transcriptional regulator -
JG531_RS12900 2558248..2558673 + 426 WP_000693943.1 Rha family transcriptional regulator -
JG531_RS12905 2558696..2559658 + 963 Protein_2519 helix-turn-helix domain-containing protein -
JG531_RS12910 2559665..2560405 + 741 WP_000788938.1 ATP-binding protein -
JG531_RS12915 2560431..2561200 + 770 Protein_2521 DUF1627 domain-containing protein -
JG531_RS12920 2561216..2561611 + 396 WP_001118159.1 DUF977 family protein -
JG531_RS12925 2561668..2562252 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2542108..2598697 56589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T184907 WP_001303511.1 NZ_CP066753:c2557266-2556988 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T184907 NZ_CP087840:c1566898-1566641 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT184907 WP_001302048.1 NZ_CP066753:c2557459-2557268 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT184907 NZ_CP087840:c1567130-1566888 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References