Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1699118..1699589 Replicon chromosome
Accession NZ_CP018252
Organism Escherichia coli strain 9000

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag BFL16_RS09270 Protein ID WP_001303511.1
Coordinates 1699118..1699396 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag BFL16_RS09275 Protein ID WP_001302048.1
Coordinates 1699398..1699589 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL16_RS09230 1694354..1694602 - 249 WP_000113189.1 excisionase -
BFL16_RS09235 1694667..1697138 - 2472 WP_072612735.1 exonuclease -
BFL16_RS09240 1697231..1697422 - 192 WP_001090200.1 DUF1482 family protein -
BFL16_RS09245 1697419..1697607 - 189 WP_000449192.1 cell division inhibition protein DicB -
BFL16_RS09250 1698008..1698172 + 165 WP_001331716.1 hypothetical protein -
BFL16_RS09255 1698176..1698394 - 219 WP_001171930.1 hypothetical protein -
BFL16_RS09260 1698466..1698765 - 300 WP_001240334.1 hypothetical protein -
BFL16_RS09270 1699118..1699396 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFL16_RS09275 1699398..1699589 - 192 WP_001302048.1 hypothetical protein Antitoxin
BFL16_RS09280 1699610..1699981 - 372 WP_001169686.1 hypothetical protein -
BFL16_RS09285 1700079..1700381 + 303 WP_000172738.1 transcriptional regulator -
BFL16_RS09290 1700378..1700803 + 426 WP_000693943.1 Rha family transcriptional regulator -
BFL16_RS09295 1700826..1701788 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
BFL16_RS09300 1701795..1702535 + 741 WP_000788938.1 ATP-binding protein -
BFL16_RS09310 1702561..1703330 + 770 Protein_1645 DUF1627 domain-containing protein -
BFL16_RS09315 1703346..1703741 + 396 WP_001118159.1 DUF977 family protein -
BFL16_RS09320 1703798..1704382 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 1693246..1740654 47408


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T69258 WP_001303511.1 NZ_CP018252:c1699396-1699118 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T69258 NZ_CP018252:c1699396-1699118 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT69258 WP_001302048.1 NZ_CP018252:c1699589-1699398 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT69258 NZ_CP018252:c1699589-1699398 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References