122933

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2877527..2877998 Replicon chromosome
Accession NZ_CP038494
Organism Escherichia coli O157:H7 strain TT12B

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E5F07_RS13940 Protein ID WP_001303511.1
Coordinates 2877720..2877998 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E5F07_RS13935 Protein ID WP_001302048.1
Coordinates 2877527..2877718 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F07_RS13895 2872734..2873318 - 585 WP_000206793.1 DUF551 domain-containing protein -
E5F07_RS13900 2873375..2873770 - 396 WP_001118159.1 DUF977 family protein -
E5F07_RS13905 2873786..2874555 - 770 Protein_2698 DUF1627 domain-containing protein -
E5F07_RS13910 2874581..2875321 - 741 WP_000788938.1 ATP-binding protein -
E5F07_RS13915 2875328..2876290 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E5F07_RS13920 2876313..2876738 - 426 WP_000693943.1 Rha family transcriptional regulator -
E5F07_RS13925 2876735..2877037 - 303 WP_000172738.1 transcriptional regulator -
E5F07_RS13930 2877135..2877506 + 372 WP_001169686.1 hypothetical protein -
E5F07_RS13935 2877527..2877718 + 192 WP_001302048.1 hypothetical protein Antitoxin
E5F07_RS13940 2877720..2877998 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E5F07_RS13945 2878351..2878650 + 300 WP_001240334.1 hypothetical protein -
E5F07_RS13950 2878722..2878940 + 219 WP_001171930.1 hypothetical protein -
E5F07_RS13960 2879509..2879697 + 189 WP_000449192.1 cell division inhibition protein DicB -
E5F07_RS13965 2879694..2879885 + 192 WP_001090200.1 DUF1482 family protein -
E5F07_RS13970 2879978..2882449 + 2472 WP_000048458.1 exonuclease -
E5F07_RS13975 2882522..2882773 + 252 WP_000005552.1 excisionase family protein -
E5F07_RS13980 2882808..2882995 + 188 Protein_2712 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2836287..2892878 56591


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122933 WP_001303511.1 NZ_CP038494:2877720-2877998 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122933 NZ_CP038494:2877720-2877998 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122933 WP_001302048.1 NZ_CP038494:2877527-2877718 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122933 NZ_CP038494:2877527-2877718 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References