Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3068071..3068542 Replicon chromosome
Accession NZ_CP038408
Organism Escherichia coli O157:H7 strain 86-24

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3169_RS15610 Protein ID WP_001303511.1
Coordinates 3068264..3068542 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3169_RS15605 Protein ID WP_001302048.1
Coordinates 3068071..3068262 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3169_RS15565 3063278..3063862 - 585 WP_000206793.1 DUF551 domain-containing protein -
E3169_RS15570 3063919..3064314 - 396 WP_001118159.1 DUF977 family protein -
E3169_RS15575 3064330..3065099 - 770 Protein_3049 DUF1627 domain-containing protein -
E3169_RS15580 3065125..3065865 - 741 WP_000788938.1 ATP-binding protein -
E3169_RS15585 3065872..3066834 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3169_RS15590 3066857..3067282 - 426 WP_000693943.1 Rha family transcriptional regulator -
E3169_RS15595 3067279..3067581 - 303 WP_000172738.1 transcriptional regulator -
E3169_RS15600 3067679..3068050 + 372 WP_001169686.1 hypothetical protein -
E3169_RS15605 3068071..3068262 + 192 WP_001302048.1 hypothetical protein Antitoxin
E3169_RS15610 3068264..3068542 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3169_RS15615 3068895..3069194 + 300 WP_001240334.1 hypothetical protein -
E3169_RS15620 3069266..3069484 + 219 WP_001171930.1 hypothetical protein -
E3169_RS15625 3069488..3069652 - 165 WP_001331716.1 hypothetical protein -
E3169_RS15630 3070053..3070241 + 189 WP_000449192.1 cell division inhibition protein DicB -
E3169_RS15635 3070238..3070429 + 192 WP_001090200.1 DUF1482 family protein -
E3169_RS15640 3070522..3072993 + 2472 WP_000048458.1 exonuclease -
E3169_RS15645 3073066..3073317 + 252 WP_000005552.1 excisionase family protein -
E3169_RS15650 3073352..3073539 + 188 Protein_3064 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 3026838..3076829 49991


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122468 WP_001303511.1 NZ_CP038408:3068264-3068542 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122468 NZ_CP038408:3068264-3068542 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122468 WP_001302048.1 NZ_CP038408:3068071-3068262 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122468 NZ_CP038408:3068071-3068262 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References