Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2817323..2817794 Replicon chromosome
Accession NZ_CP032797
Organism Escherichia coli strain ERL06-2497

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag D8Z72_RS14810 Protein ID WP_001303511.1
Coordinates 2817516..2817794 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag D8Z72_RS14805 Protein ID WP_001302048.1
Coordinates 2817323..2817514 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z72_RS14765 2812530..2813114 - 585 WP_000206793.1 DUF551 domain-containing protein -
D8Z72_RS14770 2813171..2813566 - 396 WP_001118159.1 DUF977 family protein -
D8Z72_RS14775 2813582..2814351 - 770 Protein_2822 DUF1627 domain-containing protein -
D8Z72_RS14780 2814377..2815117 - 741 WP_000788938.1 ATP-binding protein -
D8Z72_RS14785 2815124..2816086 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
D8Z72_RS14790 2816109..2816534 - 426 WP_000693943.1 Rha family transcriptional regulator -
D8Z72_RS14795 2816531..2816833 - 303 WP_000172738.1 transcriptional regulator -
D8Z72_RS14800 2816931..2817302 + 372 WP_001169686.1 hypothetical protein -
D8Z72_RS14805 2817323..2817514 + 192 WP_001302048.1 hypothetical protein Antitoxin
D8Z72_RS14810 2817516..2817794 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8Z72_RS14815 2818147..2818446 + 300 WP_001240334.1 hypothetical protein -
D8Z72_RS14820 2818518..2818736 + 219 WP_001171930.1 hypothetical protein -
D8Z72_RS14830 2819305..2819493 + 189 WP_000449192.1 cell division inhibition protein DicB -
D8Z72_RS14835 2819490..2819681 + 192 WP_001090200.1 DUF1482 family protein -
D8Z72_RS14840 2819774..2822245 + 2472 WP_000048458.1 exonuclease -
D8Z72_RS14845 2822318..2822569 + 252 WP_000005552.1 excisionase family protein -
D8Z72_RS14850 2822604..2822791 + 188 Protein_2836 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2776085..2832674 56589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T112371 WP_001303511.1 NZ_CP032797:2817516-2817794 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T112371 NZ_CP032797:2817516-2817794 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT112371 WP_001302048.1 NZ_CP032797:2817323-2817514 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT112371 NZ_CP032797:2817323-2817514 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References