Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3103896..3104367 Replicon chromosome
Accession NZ_CP062717
Organism Escherichia coli O157:H7 strain Z1825

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ56_RS15850 Protein ID WP_001303511.1
Coordinates 3104089..3104367 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ56_RS15845 Protein ID WP_001302048.1
Coordinates 3103896..3104087 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ56_RS15805 3099103..3099687 - 585 WP_000206793.1 DUF551 domain-containing protein -
ILQ56_RS15810 3099744..3100139 - 396 WP_001118159.1 DUF977 family protein -
ILQ56_RS15815 3100155..3100924 - 770 Protein_3093 DUF1627 domain-containing protein -
ILQ56_RS15820 3100950..3101690 - 741 WP_000788938.1 ATP-binding protein -
ILQ56_RS15825 3101697..3102659 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ56_RS15830 3102682..3103107 - 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ56_RS15835 3103104..3103406 - 303 WP_000172738.1 transcriptional regulator -
ILQ56_RS15840 3103504..3103875 + 372 WP_001169686.1 hypothetical protein -
ILQ56_RS15845 3103896..3104087 + 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ56_RS15850 3104089..3104367 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ56_RS15855 3104720..3105019 + 300 WP_001240334.1 hypothetical protein -
ILQ56_RS15860 3105091..3105309 + 219 WP_001171930.1 hypothetical protein -
ILQ56_RS15865 3105878..3106066 + 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ56_RS15870 3106063..3106254 + 192 WP_001090200.1 DUF1482 family protein -
ILQ56_RS15875 3106347..3108818 + 2472 WP_072612735.1 exonuclease -
ILQ56_RS15880 3108883..3109131 + 249 WP_000113189.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 3062832..3114374 51542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T175634 WP_001303511.1 NZ_CP062717:3104089-3104367 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T175634 NZ_CP082120:2053106-2053209 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT175634 WP_001302048.1 NZ_CP062717:3103896-3104087 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT175634 NZ_CP082120:c2053247-2052976 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References