Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3062228..3062699 Replicon chromosome
Accession NZ_CP062780
Organism Escherichia coli O157:H7 strain Z1769

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ49_RS15540 Protein ID WP_001303511.1
Coordinates 3062421..3062699 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ49_RS15535 Protein ID WP_001302048.1
Coordinates 3062228..3062419 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ49_RS15495 3057435..3058019 - 585 WP_000206793.1 DUF551 domain-containing protein -
ILQ49_RS15500 3058076..3058471 - 396 WP_001118159.1 DUF977 family protein -
ILQ49_RS15505 3058487..3059256 - 770 Protein_3032 DUF1627 domain-containing protein -
ILQ49_RS15510 3059282..3060022 - 741 WP_000788938.1 ATP-binding protein -
ILQ49_RS15515 3060029..3060991 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ49_RS15520 3061014..3061439 - 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ49_RS15525 3061436..3061738 - 303 WP_000172738.1 transcriptional regulator -
ILQ49_RS15530 3061836..3062207 + 372 WP_001169686.1 hypothetical protein -
ILQ49_RS15535 3062228..3062419 + 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ49_RS15540 3062421..3062699 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ49_RS15545 3063052..3063351 + 300 WP_001240334.1 hypothetical protein -
ILQ49_RS15550 3063423..3063641 + 219 WP_001171930.1 hypothetical protein -
ILQ49_RS15555 3064210..3064398 + 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ49_RS15560 3064395..3064586 + 192 WP_001090200.1 DUF1482 family protein -
ILQ49_RS15565 3064679..3067150 + 2472 WP_072612735.1 exonuclease -
ILQ49_RS15570 3067215..3067463 + 249 WP_000113189.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 3021164..3068571 47407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T176546 WP_001303511.1 NZ_CP062780:3062421-3062699 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T176546 NZ_CP082831:c4524525-4524175 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT176546 WP_001302048.1 NZ_CP062780:3062228-3062419 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT176546 NZ_CP082831:c4524770-4524519 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References