Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 5008420..5008891 Replicon chromosome
Accession NZ_CP103864
Organism Escherichia coli O157:H7 strain RV06

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag NUE47_RS24815 Protein ID WP_001303511.1
Coordinates 5008613..5008891 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag NUE47_RS24810 Protein ID WP_001302048.1
Coordinates 5008420..5008611 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE47_RS24770 (5003627) 5003627..5004211 - 585 WP_000206793.1 DUF551 domain-containing protein -
NUE47_RS24775 (5004268) 5004268..5004663 - 396 WP_001118159.1 DUF977 family protein -
NUE47_RS24780 (5004679) 5004679..5005448 - 770 Protein_4830 DUF1627 domain-containing protein -
NUE47_RS24785 (5005474) 5005474..5006214 - 741 WP_000788938.1 ATP-binding protein -
NUE47_RS24790 (5006221) 5006221..5007183 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
NUE47_RS24795 (5007206) 5007206..5007631 - 426 WP_000693943.1 toxin YdaT family protein -
NUE47_RS24800 (5007628) 5007628..5007930 - 303 WP_000172738.1 transcriptional regulator -
NUE47_RS24805 (5008028) 5008028..5008399 + 372 WP_001169686.1 hypothetical protein -
NUE47_RS24810 (5008420) 5008420..5008611 + 192 WP_001302048.1 hypothetical protein Antitoxin
NUE47_RS24815 (5008613) 5008613..5008891 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NUE47_RS24820 (5009244) 5009244..5009543 + 300 WP_001240334.1 hypothetical protein -
NUE47_RS24825 (5009615) 5009615..5009833 + 219 WP_001171930.1 protein YdfC -
NUE47_RS24830 (5010402) 5010402..5010590 + 189 WP_000449192.1 cell division inhibition protein DicB -
NUE47_RS24835 (5010587) 5010587..5010778 + 192 WP_001090200.1 DUF1482 family protein -
NUE47_RS24840 (5010871) 5010871..5013342 + 2472 WP_000048458.1 exonuclease -
NUE47_RS24845 (5013415) 5013415..5013666 + 252 WP_000005552.1 excisionase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4971060..5023771 52711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T256864 WP_001303511.1 NZ_CP103864:5008613-5008891 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT256864 WP_001302048.1 NZ_CP103864:5008420-5008611 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References