Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4063869..4064340 Replicon chromosome
Accession NZ_CP041623
Organism Escherichia coli O157:H7 strain ATCC 43888

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag FNZ21_RS20425 Protein ID WP_001303511.1
Coordinates 4064062..4064340 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag FNZ21_RS20420 Protein ID WP_001302048.1
Coordinates 4063869..4064060 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNZ21_RS20380 4059076..4059660 - 585 WP_000206793.1 DUF551 domain-containing protein -
FNZ21_RS20385 4059717..4060112 - 396 WP_001118159.1 DUF977 family protein -
FNZ21_RS20390 4060128..4060897 - 770 Protein_3880 DUF1627 domain-containing protein -
FNZ21_RS20395 4060923..4061663 - 741 WP_000788938.1 ATP-binding protein -
FNZ21_RS20400 4061670..4062632 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
FNZ21_RS20405 4062655..4063080 - 426 WP_000693943.1 Rha family transcriptional regulator -
FNZ21_RS20410 4063077..4063379 - 303 WP_000172738.1 transcriptional regulator -
FNZ21_RS20415 4063477..4063848 + 372 WP_001169686.1 hypothetical protein -
FNZ21_RS20420 4063869..4064060 + 192 WP_001302048.1 hypothetical protein Antitoxin
FNZ21_RS20425 4064062..4064340 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FNZ21_RS20430 4064693..4064992 + 300 WP_001240334.1 hypothetical protein -
FNZ21_RS20435 4065064..4065282 + 219 WP_001171930.1 hypothetical protein -
FNZ21_RS20445 4065851..4066039 + 189 WP_000449192.1 cell division inhibition protein DicB -
FNZ21_RS20450 4066036..4066227 + 192 WP_001090200.1 DUF1482 family protein -
FNZ21_RS20455 4066320..4068791 + 2472 WP_000048458.1 exonuclease -
FNZ21_RS20460 4068864..4069115 + 252 WP_000005552.1 excisionase family protein -
FNZ21_RS20465 4069150..4069337 + 188 Protein_3894 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 4022638..4079220 56582


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T128824 WP_001303511.1 NZ_CP041623:4064062-4064340 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T128824 NZ_CP041623:4064062-4064340 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT128824 WP_001302048.1 NZ_CP041623:4063869-4064060 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT128824 NZ_CP041623:4063869-4064060 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References