Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2646122..2646593 Replicon chromosome
Accession NZ_CP062746
Organism Escherichia coli O157:H7 strain Z1504

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ42_RS13450 Protein ID WP_001303511.1
Coordinates 2646122..2646400 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ42_RS13455 Protein ID WP_001302048.1
Coordinates 2646402..2646593 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ42_RS13420 2641358..2641606 - 249 WP_000113189.1 excisionase -
ILQ42_RS13425 2641671..2644142 - 2472 WP_072612735.1 exonuclease -
ILQ42_RS13430 2644235..2644426 - 192 WP_001090200.1 DUF1482 family protein -
ILQ42_RS13435 2644423..2644611 - 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ42_RS13440 2645180..2645398 - 219 WP_001171930.1 hypothetical protein -
ILQ42_RS13445 2645470..2645769 - 300 WP_001240334.1 hypothetical protein -
ILQ42_RS13450 2646122..2646400 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ42_RS13455 2646402..2646593 - 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ42_RS13460 2646614..2646985 - 372 WP_001169686.1 hypothetical protein -
ILQ42_RS13465 2647083..2647385 + 303 WP_000172738.1 transcriptional regulator -
ILQ42_RS13470 2647382..2647807 + 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ42_RS13475 2647830..2648792 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ42_RS13480 2648799..2649539 + 741 WP_000788938.1 ATP-binding protein -
ILQ42_RS13485 2649565..2650334 + 770 Protein_2635 DUF1627 domain-containing protein -
ILQ42_RS13490 2650350..2650745 + 396 WP_001118159.1 DUF977 family protein -
ILQ42_RS13495 2650802..2651386 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2636115..2687658 51543


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T176100 WP_001303511.1 NZ_CP062746:c2646400-2646122 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T176100 NZ_CP082355:c4298274-4298101 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT176100 WP_001302048.1 NZ_CP062746:c2646593-2646402 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT176100 NZ_CP082355:c4298598-4298302 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References