Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2448739..2449210 Replicon chromosome
Accession NZ_CP017444
Organism Escherichia coli O157:H7 strain 8368

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A4C39_RS12490 Protein ID WP_001303511.1
Coordinates 2448739..2449017 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A4C39_RS12495 Protein ID WP_001302048.1
Coordinates 2449019..2449210 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C39_RS12455 2443964..2444215 - 252 WP_000005552.1 excisionase family protein -
A4C39_RS12460 2444288..2446759 - 2472 WP_000048458.1 exonuclease -
A4C39_RS12465 2446852..2447043 - 192 WP_001090200.1 DUF1482 family protein -
A4C39_RS12470 2447040..2447228 - 189 WP_000449192.1 cell division inhibition protein DicB -
A4C39_RS12475 2447797..2448015 - 219 WP_001171930.1 hypothetical protein -
A4C39_RS12480 2448087..2448386 - 300 WP_001240334.1 hypothetical protein -
A4C39_RS12490 2448739..2449017 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4C39_RS12495 2449019..2449210 - 192 WP_001302048.1 hypothetical protein Antitoxin
A4C39_RS29225 2449231..2449602 - 372 WP_001169686.1 hypothetical protein -
A4C39_RS12505 2449700..2450002 + 303 WP_000172738.1 transcriptional regulator -
A4C39_RS12510 2449999..2450424 + 426 WP_000693943.1 Rha family transcriptional regulator -
A4C39_RS12515 2450447..2451409 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
A4C39_RS12520 2451416..2452156 + 741 WP_000788938.1 ATP-binding protein -
A4C39_RS12530 2452182..2452951 + 770 Protein_2377 DUF1627 domain-containing protein -
A4C39_RS12535 2452967..2453362 + 396 WP_001118159.1 DUF977 family protein -
A4C39_RS12540 2453419..2454003 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI / nleH2 2441578..2489918 48340


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T66713 WP_001303511.1 NZ_CP017444:c2449017-2448739 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T66713 NZ_CP017444:c2449017-2448739 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT66713 WP_001302048.1 NZ_CP017444:c2449210-2449019 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT66713 NZ_CP017444:c2449210-2449019 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References