Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2164796..2165267 Replicon chromosome
Accession NZ_CP032791
Organism Escherichia coli strain NZRM4165

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag D8Z75_RS10710 Protein ID WP_001303511.1
Coordinates 2164989..2165267 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag D8Z75_RS10705 Protein ID WP_001302048.1
Coordinates 2164796..2164987 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z75_RS10665 2160003..2160587 - 585 WP_000206793.1 DUF551 domain-containing protein -
D8Z75_RS10670 2160644..2161039 - 396 WP_001118159.1 DUF977 family protein -
D8Z75_RS10675 2161055..2161824 - 770 Protein_2050 DUF1627 domain-containing protein -
D8Z75_RS10680 2161850..2162590 - 741 WP_000788938.1 ATP-binding protein -
D8Z75_RS10685 2162597..2163559 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
D8Z75_RS10690 2163582..2164007 - 426 WP_000693943.1 Rha family transcriptional regulator -
D8Z75_RS10695 2164004..2164306 - 303 WP_000172738.1 transcriptional regulator -
D8Z75_RS10700 2164404..2164775 + 372 WP_001169686.1 hypothetical protein -
D8Z75_RS10705 2164796..2164987 + 192 WP_001302048.1 hypothetical protein Antitoxin
D8Z75_RS10710 2164989..2165267 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8Z75_RS10715 2165620..2165919 + 300 WP_001240334.1 hypothetical protein -
D8Z75_RS10720 2165991..2166209 + 219 WP_001171930.1 hypothetical protein -
D8Z75_RS10730 2166778..2166966 + 189 WP_000449192.1 cell division inhibition protein DicB -
D8Z75_RS10735 2166963..2167154 + 192 WP_001090200.1 DUF1482 family protein -
D8Z75_RS10740 2167247..2169718 + 2472 WP_000048458.1 exonuclease -
D8Z75_RS10745 2169791..2170042 + 252 WP_000005552.1 excisionase family protein -
D8Z75_RS10750 2170077..2170261 + 185 Protein_2064 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2124250..2173554 49304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T112262 WP_001303511.1 NZ_CP032791:2164989-2165267 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T112262 NZ_CP032791:2164989-2165267 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT112262 WP_001302048.1 NZ_CP032791:2164796-2164987 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT112262 NZ_CP032791:2164796-2164987 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References