Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3066379..3066850 Replicon chromosome
Accession NZ_CP062711
Organism Escherichia coli O157:H7 strain Z1831

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ59_RS15570 Protein ID WP_001303511.1
Coordinates 3066572..3066850 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ59_RS15565 Protein ID WP_001302048.1
Coordinates 3066379..3066570 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ59_RS15525 3061586..3062170 - 585 WP_000206793.1 DUF551 domain-containing protein -
ILQ59_RS15530 3062227..3062622 - 396 WP_001118159.1 DUF977 family protein -
ILQ59_RS15535 3062638..3063407 - 770 Protein_3038 DUF1627 domain-containing protein -
ILQ59_RS15540 3063433..3064173 - 741 WP_000788938.1 ATP-binding protein -
ILQ59_RS15545 3064180..3065142 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ59_RS15550 3065165..3065590 - 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ59_RS15555 3065587..3065889 - 303 WP_000172738.1 transcriptional regulator -
ILQ59_RS15560 3065987..3066358 + 372 WP_001169686.1 hypothetical protein -
ILQ59_RS15565 3066379..3066570 + 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ59_RS15570 3066572..3066850 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ59_RS15575 3067203..3067502 + 300 WP_001240334.1 hypothetical protein -
ILQ59_RS15580 3067574..3067792 + 219 WP_001171930.1 hypothetical protein -
ILQ59_RS15585 3068361..3068549 + 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ59_RS15590 3068546..3068737 + 192 WP_001090200.1 DUF1482 family protein -
ILQ59_RS15595 3068830..3071301 + 2472 WP_072612735.1 exonuclease -
ILQ59_RS15600 3071366..3071614 + 249 WP_000113189.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 3025139..3076857 51718


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T175526 WP_001303511.1 NZ_CP062711:3066572-3066850 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T175526 NZ_CP082019:4220837-4221055 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT175526 WP_001302048.1 NZ_CP062711:3066379-3066570 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT175526 NZ_CP082019:4220436-4220810 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References