Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2522396..2522867 Replicon chromosome
Accession NZ_CP038414
Organism Escherichia coli O157:H7 strain 17B6-2

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E4U86_RS12625 Protein ID WP_001303511.1
Coordinates 2522396..2522674 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E4U86_RS12630 Protein ID WP_001302048.1
Coordinates 2522676..2522867 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U86_RS12585 2517402..2517586 - 185 Protein_2458 DUF3596 domain-containing protein -
E4U86_RS12590 2517621..2517872 - 252 WP_000005552.1 excisionase family protein -
E4U86_RS12595 2517945..2520416 - 2472 WP_000048458.1 exonuclease -
E4U86_RS12600 2520509..2520700 - 192 WP_001090200.1 DUF1482 family protein -
E4U86_RS12605 2520697..2520885 - 189 WP_000449192.1 cell division inhibition protein DicB -
E4U86_RS12610 2521286..2521450 + 165 WP_001331716.1 hypothetical protein -
E4U86_RS12615 2521454..2521672 - 219 WP_001171930.1 hypothetical protein -
E4U86_RS12620 2521744..2522043 - 300 WP_001240334.1 hypothetical protein -
E4U86_RS12625 2522396..2522674 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U86_RS12630 2522676..2522867 - 192 WP_001302048.1 hypothetical protein Antitoxin
E4U86_RS12635 2522888..2523259 - 372 WP_001169686.1 hypothetical protein -
E4U86_RS12640 2523357..2523659 + 303 WP_000172738.1 transcriptional regulator -
E4U86_RS12645 2523656..2524081 + 426 WP_000693943.1 Rha family transcriptional regulator -
E4U86_RS12650 2524104..2525066 + 963 WP_000095668.1 helix-turn-helix domain-containing protein -
E4U86_RS12655 2525073..2525813 + 741 WP_000788938.1 ATP-binding protein -
E4U86_RS12660 2525839..2526608 + 770 Protein_2473 DUF1627 domain-containing protein -
E4U86_RS12665 2526624..2527019 + 396 WP_001118159.1 DUF977 family protein -
E4U86_RS12670 2527076..2527659 + 584 Protein_2475 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2507516..2564105 56589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122547 WP_001303511.1 NZ_CP038414:c2522674-2522396 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122547 NZ_CP038414:c2522674-2522396 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122547 WP_001302048.1 NZ_CP038414:c2522867-2522676 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122547 NZ_CP038414:c2522867-2522676 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References