Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3018688..3019159 Replicon chromosome
Accession NZ_CP017249
Organism Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag BHW76_RS15865 Protein ID WP_001303511.1
Coordinates 3018881..3019159 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag BHW76_RS15860 Protein ID WP_001302048.1
Coordinates 3018688..3018879 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW76_RS15815 3013895..3014479 - 585 WP_000206793.1 DUF551 domain-containing protein -
BHW76_RS15820 3014536..3014931 - 396 WP_001118159.1 DUF977 family protein -
BHW76_RS15825 3014947..3015716 - 770 Protein_3016 DUF1627 domain-containing protein -
BHW76_RS15835 3015742..3016482 - 741 WP_000788938.1 ATP-binding protein -
BHW76_RS15840 3016489..3017451 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
BHW76_RS15845 3017474..3017899 - 426 WP_000693943.1 Rha family transcriptional regulator -
BHW76_RS15850 3017896..3018198 - 303 WP_000172738.1 transcriptional regulator -
BHW76_RS29935 3018296..3018667 + 372 WP_001169686.1 hypothetical protein -
BHW76_RS15860 3018688..3018879 + 192 WP_001302048.1 hypothetical protein Antitoxin
BHW76_RS15865 3018881..3019159 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BHW76_RS15875 3019512..3019811 + 300 WP_001240334.1 hypothetical protein -
BHW76_RS15880 3019883..3020101 + 219 WP_001171930.1 hypothetical protein -
BHW76_RS29945 3020670..3020858 + 189 WP_000449192.1 cell division inhibition protein DicB -
BHW76_RS15885 3020855..3021046 + 192 WP_001090200.1 DUF1482 family protein -
BHW76_RS15890 3021139..3023610 + 2472 WP_000048458.1 exonuclease -
BHW76_RS15895 3023683..3023934 + 252 WP_000005552.1 excisionase family protein -
BHW76_RS31920 3023969..3024156 + 188 Protein_3030 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2977462..3034039 56577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T66301 WP_001303511.1 NZ_CP017249:3018881-3019159 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T66301 NZ_CP017249:3018881-3019159 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT66301 WP_001302048.1 NZ_CP017249:3018688-3018879 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT66301 NZ_CP017249:3018688-3018879 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References