Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1629586..1630057 Replicon chromosome
Accession NZ_CP012802
Organism Escherichia coli O157:H7 strain WS4202

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag AO055_RS08135 Protein ID WP_001303511.1
Coordinates 1629586..1629864 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AO055_RS08140 Protein ID WP_001302048.1
Coordinates 1629866..1630057 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO055_RS08100 1624811..1625062 - 252 WP_000005552.1 excisionase family protein -
AO055_RS08105 1625135..1627606 - 2472 WP_000048458.1 exonuclease -
AO055_RS08110 1627699..1627890 - 192 WP_001090200.1 DUF1482 family protein -
AO055_RS08115 1627887..1628075 - 189 WP_000449192.1 cell division inhibition protein DicB -
AO055_RS08120 1628644..1628862 - 219 WP_001171930.1 hypothetical protein -
AO055_RS08125 1628934..1629233 - 300 WP_001240334.1 hypothetical protein -
AO055_RS08135 1629586..1629864 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AO055_RS08140 1629866..1630057 - 192 WP_001302048.1 hypothetical protein Antitoxin
AO055_RS30170 1630078..1630449 - 372 WP_001169686.1 hypothetical protein -
AO055_RS08150 1630547..1630849 + 303 WP_000172738.1 transcriptional regulator -
AO055_RS08155 1630846..1631271 + 426 WP_000693943.1 Rha family transcriptional regulator -
AO055_RS08160 1631294..1632256 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
AO055_RS08165 1632263..1633003 + 741 WP_000788938.1 ATP-binding protein -
AO055_RS08175 1633029..1633798 + 770 Protein_1547 DUF1627 domain-containing protein -
AO055_RS08180 1633814..1634209 + 396 WP_001118159.1 DUF977 family protein -
AO055_RS08185 1634266..1634850 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 1622425..1671298 48873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T57224 WP_001303511.1 NZ_CP012802:c1629864-1629586 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T57224 NZ_CP012802:c1629864-1629586 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT57224 WP_001302048.1 NZ_CP012802:c1630057-1629866 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT57224 NZ_CP012802:c1630057-1629866 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References