Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2712561..2713032 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag IEM32_RS12980 Protein ID WP_001303511.1
Coordinates 2712561..2712839 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag IEM32_RS12985 Protein ID WP_001302048.1
Coordinates 2712841..2713032 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS12945 2707564..2707751 - 188 Protein_2519 DUF3596 domain-containing protein -
IEM32_RS12950 2707786..2708037 - 252 WP_000005552.1 excisionase family protein -
IEM32_RS12955 2708110..2710581 - 2472 WP_000048458.1 exonuclease -
IEM32_RS12960 2710674..2710865 - 192 WP_001090200.1 DUF1482 family protein -
IEM32_RS12965 2710862..2711050 - 189 WP_000449192.1 cell division inhibition protein DicB -
IEM32_RS12970 2711619..2711837 - 219 WP_001171930.1 hypothetical protein -
IEM32_RS12975 2711909..2712208 - 300 WP_001240334.1 hypothetical protein -
IEM32_RS12980 2712561..2712839 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IEM32_RS12985 2712841..2713032 - 192 WP_001302048.1 hypothetical protein Antitoxin
IEM32_RS12990 2713053..2713424 - 372 WP_001169686.1 hypothetical protein -
IEM32_RS12995 2713522..2713824 + 303 WP_000172738.1 transcriptional regulator -
IEM32_RS13000 2713821..2714246 + 426 WP_000693943.1 Rha family transcriptional regulator -
IEM32_RS13005 2714269..2715231 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
IEM32_RS13010 2715238..2715978 + 741 WP_000788938.1 ATP-binding protein -
IEM32_RS13015 2716004..2716773 + 770 Protein_2533 DUF1627 domain-containing protein -
IEM32_RS13020 2716789..2717184 + 396 WP_001118159.1 DUF977 family protein -
IEM32_RS13025 2717241..2717825 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2697681..2750393 52712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T173448 WP_001303511.1 NZ_CP062160:c2712839-2712561 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T173448 NZ_CP080173:147445-147861 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT173448 WP_001302048.1 NZ_CP062160:c2713032-2712841 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT173448 NZ_CP080173:147218-147448 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References