Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 34117..34588 Replicon chromosome
Accession NZ_CP034803
Organism Escherichia coli strain 2009C-3554

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A0I22_RS00200 Protein ID WP_001303511.1
Coordinates 34117..34395 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A0I22_RS00205 Protein ID WP_001302048.1
Coordinates 34397..34588 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0I22_RS00165 29342..29593 - 252 WP_000005552.1 excisionase family protein -
A0I22_RS00170 29666..32137 - 2472 WP_000048458.1 exonuclease -
A0I22_RS00175 32230..32421 - 192 WP_001090200.1 DUF1482 family protein -
A0I22_RS00180 32418..32606 - 189 WP_000449192.1 cell division inhibition protein DicB -
A0I22_RS00190 33175..33393 - 219 WP_001171930.1 hypothetical protein -
A0I22_RS00195 33465..33764 - 300 WP_001240334.1 hypothetical protein -
A0I22_RS00200 34117..34395 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A0I22_RS00205 34397..34588 - 192 WP_001302048.1 hypothetical protein Antitoxin
A0I22_RS00210 34609..34980 - 372 WP_001169686.1 hypothetical protein -
A0I22_RS00215 35078..35380 + 303 WP_000172738.1 transcriptional regulator -
A0I22_RS00220 35377..35802 + 426 WP_000693943.1 Rha family transcriptional regulator -
A0I22_RS00225 35825..36787 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
A0I22_RS00230 36794..37534 + 741 WP_000788938.1 ATP-binding protein -
A0I22_RS00235 37560..38329 + 770 Protein_45 DUF1627 domain-containing protein -
A0I22_RS00240 38345..38740 + 396 WP_001118159.1 DUF977 family protein -
A0I22_RS00245 38797..39381 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 26956..71552 44596


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T116753 WP_001303511.1 NZ_CP034803:c34395-34117 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T116753 NZ_CP034803:c34395-34117 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT116753 WP_001302048.1 NZ_CP034803:c34588-34397 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT116753 NZ_CP034803:c34588-34397 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References