Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2419270..2419741 Replicon chromosome
Accession NZ_CP040570
Organism Escherichia coli O157:H7 strain ECP17-1298

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag FGA23_RS12255 Protein ID WP_001303511.1
Coordinates 2419270..2419548 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag FGA23_RS12260 Protein ID WP_001302048.1
Coordinates 2419550..2419741 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGA23_RS12220 2414495..2414746 - 252 WP_000005552.1 excisionase family protein -
FGA23_RS12225 2414819..2417290 - 2472 WP_000048458.1 exonuclease -
FGA23_RS12230 2417383..2417574 - 192 WP_001090200.1 DUF1482 family protein -
FGA23_RS12235 2417571..2417759 - 189 WP_000449192.1 cell division inhibition protein DicB -
FGA23_RS12245 2418328..2418546 - 219 WP_001171930.1 hypothetical protein -
FGA23_RS12250 2418618..2418917 - 300 WP_001240334.1 hypothetical protein -
FGA23_RS12255 2419270..2419548 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FGA23_RS12260 2419550..2419741 - 192 WP_001302048.1 hypothetical protein Antitoxin
FGA23_RS12265 2419762..2420133 - 372 WP_001169686.1 hypothetical protein -
FGA23_RS12270 2420231..2420533 + 303 WP_000172738.1 transcriptional regulator -
FGA23_RS12275 2420530..2420955 + 426 WP_000693943.1 Rha family transcriptional regulator -
FGA23_RS12280 2420978..2421940 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
FGA23_RS12285 2421947..2422687 + 741 WP_000788938.1 ATP-binding protein -
FGA23_RS12290 2422713..2423482 + 770 Protein_2360 DUF1627 domain-containing protein -
FGA23_RS12295 2423498..2423893 + 396 WP_001118159.1 DUF977 family protein -
FGA23_RS12300 2423950..2424534 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2412109..2459227 47118


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T126049 WP_001303511.1 NZ_CP040570:c2419548-2419270 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T126049 NZ_CP040570:c2419548-2419270 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT126049 WP_001302048.1 NZ_CP040570:c2419741-2419550 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT126049 NZ_CP040570:c2419741-2419550 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References