Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3089934..3090405 Replicon chromosome
Accession NZ_CP028652
Organism Escherichia coli strain 123

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E52_RS16300 Protein ID WP_001303511.1
Coordinates 3090127..3090405 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E52_RS16295 Protein ID WP_001302048.1
Coordinates 3089934..3090125 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E52_RS16255 3085141..3085725 - 585 WP_000206793.1 DUF551 domain-containing protein -
C9E52_RS16260 3085782..3086177 - 396 WP_001118159.1 DUF977 family protein -
C9E52_RS16265 3086193..3086962 - 770 Protein_3091 DUF1627 domain-containing protein -
C9E52_RS16270 3086988..3087728 - 741 WP_000788938.1 ATP-binding protein -
C9E52_RS16275 3087735..3088697 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E52_RS16280 3088720..3089145 - 426 WP_000693943.1 Rha family transcriptional regulator -
C9E52_RS16285 3089142..3089444 - 303 WP_000172738.1 transcriptional regulator -
C9E52_RS16290 3089542..3089913 + 372 WP_001169686.1 hypothetical protein -
C9E52_RS16295 3089934..3090125 + 192 WP_001302048.1 hypothetical protein Antitoxin
C9E52_RS16300 3090127..3090405 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E52_RS16305 3090758..3091057 + 300 WP_001240334.1 hypothetical protein -
C9E52_RS16310 3091129..3091347 + 219 WP_001171930.1 hypothetical protein -
C9E52_RS16320 3091916..3092104 + 189 WP_000449192.1 cell division inhibition protein DicB -
C9E52_RS16325 3092101..3092292 + 192 WP_001090200.1 DUF1482 family protein -
C9E52_RS16330 3092385..3094856 + 2472 WP_000048458.1 exonuclease -
C9E52_RS16335 3094929..3095180 + 252 WP_000005552.1 excisionase family protein -
C9E52_RS16340 3095215..3095402 + 188 Protein_3105 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 3050611..3097566 46955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T102300 WP_001303511.1 NZ_CP028652:3090127-3090405 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T102300 NZ_CP028652:3090127-3090405 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT102300 WP_001302048.1 NZ_CP028652:3089934-3090125 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT102300 NZ_CP028652:3089934-3090125 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References