Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3018698..3019169 Replicon chromosome
Accession NZ_CP017251
Organism Escherichia coli strain NADC 5570/86-24/6564 isolate wild

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag BHW77_RS15880 Protein ID WP_001303511.1
Coordinates 3018891..3019169 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag BHW77_RS15875 Protein ID WP_001302048.1
Coordinates 3018698..3018889 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW77_RS15830 3013905..3014489 - 585 WP_000206793.1 DUF551 domain-containing protein -
BHW77_RS15835 3014546..3014941 - 396 WP_001118159.1 DUF977 family protein -
BHW77_RS15840 3014957..3015726 - 770 Protein_3013 DUF1627 domain-containing protein -
BHW77_RS15850 3015752..3016492 - 741 WP_000788938.1 ATP-binding protein -
BHW77_RS15855 3016499..3017461 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
BHW77_RS15860 3017484..3017909 - 426 WP_000693943.1 Rha family transcriptional regulator -
BHW77_RS15865 3017906..3018208 - 303 WP_000172738.1 transcriptional regulator -
BHW77_RS29925 3018306..3018677 + 372 WP_001169686.1 hypothetical protein -
BHW77_RS15875 3018698..3018889 + 192 WP_001302048.1 hypothetical protein Antitoxin
BHW77_RS15880 3018891..3019169 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BHW77_RS15890 3019522..3019821 + 300 WP_001240334.1 hypothetical protein -
BHW77_RS15895 3019893..3020111 + 219 WP_001171930.1 hypothetical protein -
BHW77_RS29935 3020680..3020868 + 189 WP_000449192.1 cell division inhibition protein DicB -
BHW77_RS15900 3020865..3021056 + 192 WP_001090200.1 DUF1482 family protein -
BHW77_RS15905 3021149..3023620 + 2472 WP_000048458.1 exonuclease -
BHW77_RS15910 3023693..3023944 + 252 WP_000005552.1 excisionase family protein -
BHW77_RS31925 3023979..3024166 + 188 Protein_3027 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2977472..3034049 56577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T66333 WP_001303511.1 NZ_CP017251:3018891-3019169 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T66333 NZ_CP017251:3018891-3019169 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT66333 WP_001302048.1 NZ_CP017251:3018698-3018889 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT66333 NZ_CP017251:3018698-3018889 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References