Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2420394..2420865 Replicon chromosome
Accession NZ_CP031913
Organism Escherichia coli O157:H7 strain FWSEC0004

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag CAM50_RS12275 Protein ID WP_001303511.1
Coordinates 2420394..2420672 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag CAM50_RS12280 Protein ID WP_001302048.1
Coordinates 2420674..2420865 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAM50_RS12240 2415619..2415870 - 252 WP_000005552.1 excisionase family protein -
CAM50_RS12245 2415943..2418414 - 2472 WP_000048458.1 exonuclease -
CAM50_RS12250 2418507..2418698 - 192 WP_001090200.1 DUF1482 family protein -
CAM50_RS12255 2418695..2418883 - 189 WP_000449192.1 cell division inhibition protein DicB -
CAM50_RS12265 2419452..2419670 - 219 WP_001171930.1 hypothetical protein -
CAM50_RS12270 2419742..2420041 - 300 WP_001240334.1 hypothetical protein -
CAM50_RS12275 2420394..2420672 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CAM50_RS12280 2420674..2420865 - 192 WP_001302048.1 hypothetical protein Antitoxin
CAM50_RS12285 2420886..2421257 - 372 WP_001169686.1 hypothetical protein -
CAM50_RS12290 2421355..2421657 + 303 WP_000172738.1 transcriptional regulator -
CAM50_RS12295 2421654..2422079 + 426 WP_000693943.1 Rha family transcriptional regulator -
CAM50_RS12300 2422102..2423064 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
CAM50_RS12305 2423071..2423811 + 741 WP_000788938.1 ATP-binding protein -
CAM50_RS12310 2423837..2424606 + 770 Protein_2362 DUF1627 domain-containing protein -
CAM50_RS12315 2424622..2425017 + 396 WP_001118159.1 DUF977 family protein -
CAM50_RS12320 2425074..2425658 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2405514..2462082 56568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T110704 WP_001303511.1 NZ_CP031913:c2420672-2420394 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T110704 NZ_CP031913:c2420672-2420394 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT110704 WP_001302048.1 NZ_CP031913:c2420865-2420674 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT110704 NZ_CP031913:c2420865-2420674 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References