Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2343375..2343846 Replicon chromosome
Accession NZ_CP008957
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag EDL933_RS12330 Protein ID WP_001303511.1
Coordinates 2343568..2343846 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EDL933_RS12325 Protein ID WP_001302048.1
Coordinates 2343375..2343566 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDL933_RS12280 2338582..2339166 - 585 WP_000206793.1 DUF551 domain-containing protein -
EDL933_RS12285 2339223..2339618 - 396 WP_001118159.1 DUF977 family protein -
EDL933_RS12290 2339634..2340403 - 770 Protein_2334 DUF1627 domain-containing protein -
EDL933_RS12300 2340429..2341169 - 741 WP_000788938.1 ATP-binding protein -
EDL933_RS12305 2341176..2342138 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
EDL933_RS12310 2342161..2342586 - 426 WP_000693943.1 Rha family transcriptional regulator -
EDL933_RS12315 2342583..2342885 - 303 WP_000172738.1 transcriptional regulator -
EDL933_RS32475 2342983..2343354 + 372 WP_001169686.1 hypothetical protein -
EDL933_RS12325 2343375..2343566 + 192 WP_001302048.1 hypothetical protein Antitoxin
EDL933_RS12330 2343568..2343846 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EDL933_RS12340 2344199..2344498 + 300 WP_001240334.1 hypothetical protein -
EDL933_RS12345 2344570..2344788 + 219 WP_001171930.1 hypothetical protein -
EDL933_RS30650 2345357..2345545 + 189 WP_000449192.1 cell division inhibition protein DicB -
EDL933_RS12350 2345542..2345733 + 192 WP_001090200.1 DUF1482 family protein -
EDL933_RS12355 2345826..2348297 + 2472 WP_000048458.1 exonuclease -
EDL933_RS12360 2348370..2348621 + 252 WP_000005552.1 excisionase family protein -
EDL933_RS33510 2348656..2348840 + 185 Protein_2348 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2292176..2358726 66550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T47982 WP_001303511.1 NZ_CP008957:2343568-2343846 [Escherichia coli O157:H7 str. EDL933]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T47982 NZ_CP008957:2343568-2343846 [Escherichia coli O157:H7 str. EDL933]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT47982 WP_001302048.1 NZ_CP008957:2343375-2343566 [Escherichia coli O157:H7 str. EDL933]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT47982 NZ_CP008957:2343375-2343566 [Escherichia coli O157:H7 str. EDL933]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References