Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2989031..2989502 Replicon chromosome
Accession NZ_CP038496
Organism Escherichia coli O157:H7 strain TT12A

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E5F08_RS14705 Protein ID WP_001303511.1
Coordinates 2989224..2989502 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E5F08_RS14700 Protein ID WP_001302048.1
Coordinates 2989031..2989222 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F08_RS14660 2984238..2984822 - 585 WP_000206793.1 DUF551 domain-containing protein -
E5F08_RS14665 2984879..2985274 - 396 WP_001118159.1 DUF977 family protein -
E5F08_RS14670 2985290..2986059 - 770 Protein_2853 DUF1627 domain-containing protein -
E5F08_RS14675 2986085..2986825 - 741 WP_000788938.1 ATP-binding protein -
E5F08_RS14680 2986832..2987794 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E5F08_RS14685 2987817..2988242 - 426 WP_000693943.1 Rha family transcriptional regulator -
E5F08_RS14690 2988239..2988541 - 303 WP_000172738.1 transcriptional regulator -
E5F08_RS14695 2988639..2989010 + 372 WP_001169686.1 hypothetical protein -
E5F08_RS14700 2989031..2989222 + 192 WP_001302048.1 hypothetical protein Antitoxin
E5F08_RS14705 2989224..2989502 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E5F08_RS14710 2989855..2990154 + 300 WP_001240334.1 hypothetical protein -
E5F08_RS14715 2990226..2990444 + 219 WP_001171930.1 hypothetical protein -
E5F08_RS14720 2991013..2991201 + 189 WP_000449192.1 cell division inhibition protein DicB -
E5F08_RS14725 2991198..2991389 + 192 WP_001090200.1 DUF1482 family protein -
E5F08_RS14730 2991482..2993953 + 2472 WP_000048458.1 exonuclease -
E5F08_RS14735 2994026..2994277 + 252 WP_000005552.1 excisionase family protein -
E5F08_RS14740 2994312..2994496 + 185 Protein_2867 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2947793..2994467 46674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122964 WP_001303511.1 NZ_CP038496:2989224-2989502 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122964 NZ_CP038496:2989224-2989502 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122964 WP_001302048.1 NZ_CP038496:2989031-2989222 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122964 NZ_CP038496:2989031-2989222 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References