Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1701704..1702175 Replicon chromosome
Accession NZ_CP018237
Organism Escherichia coli strain 155

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag BFL20_RS09285 Protein ID WP_001303511.1
Coordinates 1701704..1701982 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag BFL20_RS09290 Protein ID WP_001302048.1
Coordinates 1701984..1702175 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL20_RS09245 1696940..1697188 - 249 WP_000113189.1 excisionase -
BFL20_RS09250 1697253..1699724 - 2472 WP_072612735.1 exonuclease -
BFL20_RS09255 1699817..1700008 - 192 WP_001090200.1 DUF1482 family protein -
BFL20_RS09260 1700005..1700193 - 189 WP_000449192.1 cell division inhibition protein DicB -
BFL20_RS09270 1700762..1700980 - 219 WP_001171930.1 hypothetical protein -
BFL20_RS09275 1701052..1701351 - 300 WP_001240334.1 hypothetical protein -
BFL20_RS09285 1701704..1701982 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFL20_RS09290 1701984..1702175 - 192 WP_001302048.1 hypothetical protein Antitoxin
BFL20_RS09295 1702196..1702567 - 372 WP_001169686.1 hypothetical protein -
BFL20_RS09300 1702665..1702967 + 303 WP_000172738.1 transcriptional regulator -
BFL20_RS09305 1702964..1703389 + 426 WP_000693943.1 Rha family transcriptional regulator -
BFL20_RS09310 1703412..1704374 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
BFL20_RS09315 1704381..1705121 + 741 WP_000788938.1 ATP-binding protein -
BFL20_RS09325 1705147..1705916 + 770 Protein_1640 DUF1627 domain-containing protein -
BFL20_RS09330 1705932..1706327 + 396 WP_001118159.1 DUF977 family protein -
BFL20_RS09335 1706384..1706968 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 1691697..1743242 51545


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T68975 WP_001303511.1 NZ_CP018237:c1701982-1701704 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T68975 NZ_CP018237:c1701982-1701704 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT68975 WP_001302048.1 NZ_CP018237:c1702175-1701984 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT68975 NZ_CP018237:c1702175-1701984 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References