Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2649994..2650465 Replicon chromosome
Accession NZ_CP062744
Organism Escherichia coli O157:H7 strain Z1615

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ43_RS13480 Protein ID WP_001303511.1
Coordinates 2649994..2650272 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ43_RS13485 Protein ID WP_001302048.1
Coordinates 2650274..2650465 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ43_RS13450 2645230..2645478 - 249 WP_000113189.1 excisionase -
ILQ43_RS13455 2645543..2648014 - 2472 WP_072612735.1 exonuclease -
ILQ43_RS13460 2648107..2648298 - 192 WP_001090200.1 DUF1482 family protein -
ILQ43_RS13465 2648295..2648483 - 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ43_RS13470 2649052..2649270 - 219 WP_001171930.1 hypothetical protein -
ILQ43_RS13475 2649342..2649641 - 300 WP_001240334.1 hypothetical protein -
ILQ43_RS13480 2649994..2650272 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ43_RS13485 2650274..2650465 - 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ43_RS13490 2650486..2650857 - 372 WP_001169686.1 hypothetical protein -
ILQ43_RS13495 2650955..2651257 + 303 WP_000172738.1 transcriptional regulator -
ILQ43_RS13500 2651254..2651679 + 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ43_RS13505 2651702..2652664 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ43_RS13510 2652671..2653411 + 741 WP_000788938.1 ATP-binding protein -
ILQ43_RS13515 2653437..2654206 + 770 Protein_2640 DUF1627 domain-containing protein -
ILQ43_RS13520 2654222..2654617 + 396 WP_001118159.1 DUF977 family protein -
ILQ43_RS13525 2654674..2655258 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 2642338..2691528 49190


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T176062 WP_001303511.1 NZ_CP062744:c2650272-2649994 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T176062 NZ_CP082349:1857698-1857952 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT176062 WP_001302048.1 NZ_CP062744:c2650465-2650274 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT176062 NZ_CP082349:1857450-1857701 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References