Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2417602..2418073 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag NUE46_RS12060 Protein ID WP_001303511.1
Coordinates 2417602..2417880 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag NUE46_RS12065 Protein ID WP_001302048.1
Coordinates 2417882..2418073 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS12030 (2412827) 2412827..2413078 - 252 WP_000005552.1 excisionase family protein -
NUE46_RS12035 (2413151) 2413151..2415622 - 2472 WP_000048458.1 exonuclease -
NUE46_RS12040 (2415715) 2415715..2415906 - 192 WP_001090200.1 DUF1482 family protein -
NUE46_RS12045 (2415903) 2415903..2416091 - 189 WP_000449192.1 cell division inhibition protein DicB -
NUE46_RS12050 (2416660) 2416660..2416878 - 219 WP_001171930.1 protein YdfC -
NUE46_RS12055 (2416950) 2416950..2417249 - 300 WP_001240334.1 hypothetical protein -
NUE46_RS12060 (2417602) 2417602..2417880 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NUE46_RS12065 (2417882) 2417882..2418073 - 192 WP_001302048.1 hypothetical protein Antitoxin
NUE46_RS12070 (2418094) 2418094..2418465 - 372 WP_001169686.1 hypothetical protein -
NUE46_RS12075 (2418563) 2418563..2418865 + 303 WP_000172738.1 transcriptional regulator -
NUE46_RS12080 (2418862) 2418862..2419287 + 426 WP_000693943.1 toxin YdaT family protein -
NUE46_RS12085 (2419310) 2419310..2420272 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
NUE46_RS12090 (2420279) 2420279..2421019 + 741 WP_000788938.1 ATP-binding protein -
NUE46_RS12095 (2421045) 2421045..2421814 + 770 Protein_2366 DUF1627 domain-containing protein -
NUE46_RS12100 (2421830) 2421830..2422225 + 396 WP_001118159.1 DUF977 family protein -
NUE46_RS12105 (2422282) 2422282..2422866 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2402722..2455433 52711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T257539 WP_001303511.1 NZ_CP104309:c2417880-2417602 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT257539 WP_001302048.1 NZ_CP104309:c2418073-2417882 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References