Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 314440..314911 Replicon chromosome
Accession NZ_CP043539
Organism Escherichia coli O157 strain Al Ain

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag F0L19_RS01580 Protein ID WP_001303511.1
Coordinates 314440..314718 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag F0L19_RS01585 Protein ID WP_001302048.1
Coordinates 314720..314911 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0L19_RS01540 309446..309630 - 185 Protein_300 DUF3596 domain-containing protein -
F0L19_RS01545 309665..309916 - 252 WP_000005552.1 excisionase family protein -
F0L19_RS01550 309989..312460 - 2472 WP_000048458.1 exonuclease -
F0L19_RS01555 312553..312744 - 192 WP_001090200.1 DUF1482 family protein -
F0L19_RS01560 312741..312929 - 189 WP_000449192.1 cell division inhibition protein DicB -
F0L19_RS01570 313498..313716 - 219 WP_001171930.1 hypothetical protein -
F0L19_RS01575 313788..314087 - 300 WP_001240334.1 hypothetical protein -
F0L19_RS01580 314440..314718 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F0L19_RS01585 314720..314911 - 192 WP_001302048.1 hypothetical protein Antitoxin
F0L19_RS01590 314932..315303 - 372 WP_001169686.1 hypothetical protein -
F0L19_RS01595 315401..315703 + 303 WP_000172738.1 transcriptional regulator -
F0L19_RS01600 315700..316124 + 425 Protein_311 Rha family transcriptional regulator -
F0L19_RS01605 316147..317109 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
F0L19_RS01610 317116..317856 + 741 WP_000788938.1 ATP-binding protein -
F0L19_RS01615 317882..318651 + 770 Protein_314 DUF1627 domain-containing protein -
F0L19_RS01620 318667..319062 + 396 WP_001118159.1 DUF977 family protein -
F0L19_RS01625 319119..319703 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI 306530..356138 49608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T138939 WP_001303511.1 NZ_CP043539:c314718-314440 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T138939 NZ_CP058305:100232-100438 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT138939 WP_001302048.1 NZ_CP043539:c314911-314720 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT138939 NZ_CP058305:c100245-100184 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References