10128

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/-
Location 3406188..3406868 Replicon chromosome
Accession NZ_NQJO01000001
Organism Erwinia amylovora strain CTBT3-1 tig00000000

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag CS942_RS16100 Protein ID WP_004155189.1
Coordinates 3406188..3406508 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag CS942_RS16105 Protein ID WP_004155188.1
Coordinates 3406545..3406868 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS942_RS16080 3403999..3404199 - 201 Protein_3033 transposase -
CS942_RS16085 3404618..3404893 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
CS942_RS16090 3404897..3405145 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
CS942_RS16095 3405242..3406075 - 834 WP_004155190.1 DUF4942 domain-containing protein -
CS942_RS16100 3406188..3406508 - 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
CS942_RS16105 3406545..3406868 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CS942_RS16110 3406881..3407351 - 471 WP_004155187.1 DNA repair protein RadC -
CS942_RS16115 3407422..3407996 - 575 Protein_3040 DUF932 domain-containing protein -
CS942_RS16120 3408221..3408463 - 243 WP_004155184.1 hypothetical protein -
CS942_RS16130 3409673..3409870 + 198 WP_004155182.1 AlpA family phage regulatory protein -
CS942_RS19830 3409978..3410572 + 595 Protein_3043 hypothetical protein -
CS942_RS16140 3410772..3411857 + 1086 WP_004155179.1 DNA sulfur modification protein DndB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3404618..3427747 23129
flank IS/Tn - - 3403975..3404199 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T10128 WP_004155189.1 NZ_NQJO01000001:c3406508-3406188 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T10128 NZ_NQJO01000001:c3406508-3406188 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT10128 WP_004155188.1 NZ_NQJO01000001:c3406868-3406545 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT10128 NZ_NQJO01000001:c3406868-3406545 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10198 Escherichia coli str. K-12 substr. MG1655

72.115

98.113

0.708
T10199 Escherichia coli str. K-12 substr. MG1655

67.619

99.057

0.67
T1042 Escherichia coli str. K-12 substr. MG1655

63.043

86.792

0.547

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10198 Escherichia coli str. K-12 substr. MG1655

64.948

92.381

0.6
AT10199 Escherichia coli str. K-12 substr. MG1655

62.245

93.333

0.581
AT1042 Escherichia coli str. K-12 substr. MG1655

62.626

92.523

0.579

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References

(1) Teja Shidore et al. (2019) Survey of Toxin⁻Antitoxin Systems in Erwinia amylovora Reveals Insights into Diversity and Functional Specificity. Toxins 11(4):206. [PubMed:30959879] experimental


experimental experimental literature