152419

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3302109..3302789 Replicon chromosome
Accession NZ_CP050244
Organism Erwinia amylovora strain TS3238

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag EHX00_RS14925 Protein ID WP_004155189.1
Coordinates 3302109..3302429 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag EHX00_RS14930 Protein ID WP_004155188.1
Coordinates 3302466..3302789 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHX00_RS14900 3299772..3299924 - 153 WP_004163933.1 hypothetical protein -
EHX00_RS14905 3299920..3300120 - 201 Protein_2870 transposase -
EHX00_RS14910 3300539..3300814 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
EHX00_RS14915 3300818..3301066 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
EHX00_RS14920 3301163..3301996 - 834 WP_004155190.1 DUF4942 domain-containing protein -
EHX00_RS14925 3302109..3302429 - 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
EHX00_RS14930 3302466..3302789 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EHX00_RS14935 3302802..3303272 - 471 WP_004155187.1 DNA repair protein RadC -
EHX00_RS14940 3303343..3303917 - 575 Protein_2877 DUF932 domain-containing protein -
EHX00_RS14945 3304142..3304384 - 243 WP_004155184.1 hypothetical protein -
EHX00_RS14950 3305594..3305791 + 198 WP_004155182.1 AlpA family phage regulatory protein -
EHX00_RS14955 3305899..3306493 + 595 Protein_2880 hypothetical protein -
EHX00_RS14960 3306693..3307778 + 1086 WP_004155179.1 DNA sulfur modification protein DndB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3300539..3323710 23171
flank IS/Tn - - 3299896..3300120 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T152419 WP_004155189.1 NZ_CP050244:c3302429-3302109 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T152419 NZ_CP066144:c4916048-4915737 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT152419 WP_004155188.1 NZ_CP050244:c3302789-3302466 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT152419 NZ_CP066144:c4915736-4915446 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References