Detailed information of TA system TA010199

Experimentally validated

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/-
• Location: 2777115..2777782
• Replicon: chromosome
• Accession: NC_000913
• Organism: Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: Q46953
• Locus tag: b2646
• Protein ID: NP_417133.1
• Coordinates: 2777453..2777782 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: P52141
• Locus tag: b2645
• Protein ID: NP_417132.1
• Coordinates: 2777115..2777432 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
b2642	2773318..2775021	+	1704	NP_417129.1	uncharacterized protein YfjW	-
b2643	2775919..2776377	+	459	NP_417130.1	putative antirestriction protein YfjX	-
b2644	2776386..2776868	+	483	NP_417131.1	RadC-like JAB domain-containing protein YfjY	-
b4548	2776877..2777077	+	201	YP_588466.1	DUF987 domain-containing protein YpjJ	-
b2645	2777115..2777432	+	318	NP_417132.1	putative antitoxin of the YpjF-YfjZ toxin-antitoxin system	Antitoxin
b2646	2777453..2777782	+	330	NP_417133.1	toxin of the YpjF-YfjZ toxin-antitoxin system	Toxin
b2647	2778146..2782726	-	4581	NP_417134.2	adhesin-like autotransporter YpjA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12308.21 Da
• Isoelectric Point: 7.2761

>T10199 NP_417133.1 NC_000913:2777453-2777782 [Escherichia coli str. K-12 substr. MG1655]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T10199 NC_000913:2777453-2777782 [Escherichia coli str. K-12 substr. MG1655]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11737.27 Da
• Isoelectric Point: 6.4630

>AT10199 NP_417132.1 NC_000913:2777115-2777432 [Escherichia coli str. K-12 substr. MG1655]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT10199 NC_000913:2777115-2777432 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10198	Escherichia coli str. K-12 substr. MG1655	80.952	96.33	0.78
T10128	Erwinia amylovora strain CTBT3-1 tig00000000	67.619	99.057	0.67
T1042	Escherichia coli str. K-12 substr. MG1655	65.385	95.413	0.624

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10198	Escherichia coli str. K-12 substr. MG1655	83.81	100	0.838
AT1042	Escherichia coli str. K-12 substr. MG1655	66.327	93.333	0.619
AT10128	Erwinia amylovora strain CTBT3-1 tig00000000	62.245	93.333	0.581

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: Q46953

Antitoxin

• AlphaFold DB: P52141

References

(1) Zhongling Wen et al. (2017) Interaction of Type IV Toxin/Antitoxin Systems in Cryptic Prophages of Escherichia coli K-12. Toxins 9(3):77. [PubMed:28257056]


 experimental literature