Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yfjZ-ypjF/- |
| Location | 2777115..2777782 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | ypjF | Uniprot ID | Q46953 |
| Locus tag | b2646 | Protein ID | NP_417133.1 |
| Coordinates | 2777453..2777782 (+) | Length | 110 a.a. |
Antitoxin (Protein)
| Gene name | yfjZ | Uniprot ID | P52141 |
| Locus tag | b2645 | Protein ID | NP_417132.1 |
| Coordinates | 2777115..2777432 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b2642 | 2773318..2775021 | + | 1704 | NP_417129.1 | uncharacterized protein YfjW | - |
| b2643 | 2775919..2776377 | + | 459 | NP_417130.1 | putative antirestriction protein YfjX | - |
| b2644 | 2776386..2776868 | + | 483 | NP_417131.1 | RadC-like JAB domain-containing protein YfjY | - |
| b4548 | 2776877..2777077 | + | 201 | YP_588466.1 | DUF987 domain-containing protein YpjJ | - |
| b2645 | 2777115..2777432 | + | 318 | NP_417132.1 | putative antitoxin of the YpjF-YfjZ toxin-antitoxin system | Antitoxin |
| b2646 | 2777453..2777782 | + | 330 | NP_417133.1 | toxin of the YpjF-YfjZ toxin-antitoxin system | Toxin |
| b2647 | 2778146..2782726 | - | 4581 | NP_417134.2 | adhesin-like autotransporter YpjA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 110 a.a. Molecular weight: 12308.21 Da Isoelectric Point: 7.2761
>T10199 NP_417133.1 NC_000913:2777453-2777782 [Escherichia coli str. K-12 substr. MG1655]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK
Download Length: 330 bp
>T10199 NC_000913:2777453-2777782 [Escherichia coli str. K-12 substr. MG1655]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11737.27 Da Isoelectric Point: 6.4630
>AT10199 NP_417132.1 NC_000913:2777115-2777432 [Escherichia coli str. K-12 substr. MG1655]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR
Download Length: 318 bp
>AT10199 NC_000913:2777115-2777432 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10198 | Escherichia coli str. K-12 substr. MG1655 |
80.952 |
96.33 |
0.78 |
| T10128 | Erwinia amylovora strain CTBT3-1 tig00000000 |
67.619 |
99.057 |
0.67 |
| T1042 | Escherichia coli str. K-12 substr. MG1655 |
65.385 |
95.413 |
0.624 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10198 | Escherichia coli str. K-12 substr. MG1655 |
83.81 |
100 |
0.838 |
| AT1042 | Escherichia coli str. K-12 substr. MG1655 |
66.327 |
93.333 |
0.619 |
| AT10128 | Erwinia amylovora strain CTBT3-1 tig00000000 |
62.245 |
93.333 |
0.581 |
Multiple sequence alignment
References
(1) Zhongling Wen et al. (2017) Interaction of Type IV Toxin/Antitoxin Systems in Cryptic Prophages of Escherichia coli K-12. Toxins 9(3):77. [PubMed:28257056]