Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/- |
| Location | 2075136..2075967 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | P64524 |
| Locus tag | b2005 | Protein ID | NP_416509.1 |
| Coordinates | 2077569..2077943 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | P76364 |
| Locus tag | b2004 | Protein ID | NP_416508.1 |
| Coordinates | 2077112..2077480 (+) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b2000 | 2071539..2074658 | + | 3120 | YP_026164.1 | self recognizing antigen 43 (Ag43) autotransporter | - |
| b2001 | 2074779..2076311 | + | 1533 | NP_416505.2 | inner membrane protein YeeR | - |
| b2002 | 2076308..2076754 | + | 447 | NP_416506.1 | RadC-like JAB domain-containing protein YeeS | - |
| b2003 | 2076817..2077038 | + | 222 | NP_416507.1 | DUF987 domain-containing protein YeeT | - |
| b2004 | 2077112..2077480 | + | 369 | NP_416508.1 | cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| b2005 | 2077569..2077943 | + | 375 | NP_416509.1 | cytoskeleton-binding toxin CbtA | Toxin |
| b2006 | 2077940..2078134 | + | 195 | YP_009518797.1 | putative uncharacterized protein YeeW | - |
| b4538 | 2078575..2078931 | + | 357 | YP_009518798.1 | putative uncharacterized protein YoeF | - |
| b2007 | 2079032..2079361 | - | 330 | NP_416511.4 | PTS system regulator TmaR | - |
| b4818 | 2079427..2079477 | - | 51 | YP_010283912.1 | protein YoeJ | - |
| b2008 | 2079533..2080591 | - | 1059 | NP_416512.1 | putative transporter YeeA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13898.94 Da Isoelectric Point: 7.8522
>T1042 NP_416509.1 NC_000913:2077569-2077943 [Escherichia coli str. K-12 substr. MG1655]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T1042 NC_000913:2077569-2077943 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13651.52 Da Isoelectric Point: 5.9541
>AT1042 NP_416508.1 NC_000913:2077112-2077480 [Escherichia coli str. K-12 substr. MG1655]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN
Download Length: 369 bp
>AT1042 NC_000913:2077112-2077480 [Escherichia coli str. K-12 substr. MG1655]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10199 | Escherichia coli str. K-12 substr. MG1655 |
65.385 |
95.413 |
0.624 |
| T10198 | Escherichia coli str. K-12 substr. MG1655 |
60 |
97.345 |
0.584 |
| T10128 | Erwinia amylovora strain CTBT3-1 tig00000000 |
63.043 |
86.792 |
0.547 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10199 | Escherichia coli str. K-12 substr. MG1655 |
66.327 |
93.333 |
0.619 |
| AT10198 | Escherichia coli str. K-12 substr. MG1655 |
62 |
95.238 |
0.59 |
| AT10128 | Erwinia amylovora strain CTBT3-1 tig00000000 |
62.626 |
92.523 |
0.579 |
Multiple sequence alignment
References
(1) Hisako Masuda et al. (2012) YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli. Molecular Microbiology 84(5):979-89. [PubMed:22515815]