152409

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3301902..3302582 Replicon chromosome
Accession NZ_CP050242
Organism Erwinia amylovora strain FB-307

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag EHW99_RS14950 Protein ID WP_004155189.1
Coordinates 3301902..3302222 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag EHW99_RS14955 Protein ID WP_004155188.1
Coordinates 3302259..3302582 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHW99_RS14925 3299565..3299717 - 153 WP_004163933.1 hypothetical protein -
EHW99_RS14930 3299713..3299913 - 201 Protein_2875 transposase -
EHW99_RS14935 3300332..3300607 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
EHW99_RS14940 3300611..3300859 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
EHW99_RS14945 3300956..3301789 - 834 WP_004155190.1 DUF4942 domain-containing protein -
EHW99_RS14950 3301902..3302222 - 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
EHW99_RS14955 3302259..3302582 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EHW99_RS14960 3302595..3303065 - 471 WP_004155187.1 DNA repair protein RadC -
EHW99_RS14965 3303136..3303710 - 575 Protein_2882 DUF932 domain-containing protein -
EHW99_RS14970 3303935..3304177 - 243 WP_004155184.1 hypothetical protein -
EHW99_RS14975 3305387..3305584 + 198 WP_004155182.1 AlpA family phage regulatory protein -
EHW99_RS14980 3305692..3306286 + 595 Protein_2885 hypothetical protein -
EHW99_RS14985 3306486..3307571 + 1086 WP_004155179.1 DNA sulfur modification protein DndB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3300332..3323509 23177
flank IS/Tn - - 3299689..3299913 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T152409 WP_004155189.1 NZ_CP050242:c3302222-3301902 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T152409 NZ_CP066144:2998688-2998966 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT152409 WP_004155188.1 NZ_CP050242:c3302582-3302259 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT152409 NZ_CP066144:2998495-2998686 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References