Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 244455..245135 Replicon chromosome
Accession NZ_CP076590
Organism Erwinia amylovora isolate CP200930

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag KQH39_RS01225 Protein ID WP_004155189.1
Coordinates 244815..245135 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag KQH39_RS01220 Protein ID WP_004155188.1
Coordinates 244455..244778 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH39_RS01190 (KQH39_01200) 239466..240551 - 1086 WP_004155179.1 DNA sulfur modification protein DndB -
KQH39_RS01195 240995..241345 - 351 WP_004155181.1 hypothetical protein -
KQH39_RS01200 (KQH39_01210) 241453..241650 - 198 WP_004155182.1 AlpA family phage regulatory protein -
KQH39_RS01205 (KQH39_01215) 242860..243102 + 243 WP_004155184.1 hypothetical protein -
KQH39_RS01210 (KQH39_01220) 243327..243901 + 575 Protein_232 DUF932 domain-containing protein -
KQH39_RS01215 (KQH39_01225) 243972..244442 + 471 WP_004155187.1 DNA repair protein RadC -
KQH39_RS01220 (KQH39_01230) 244455..244778 + 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KQH39_RS01225 (KQH39_01235) 244815..245135 + 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
KQH39_RS01230 (KQH39_01240) 245248..246081 + 834 WP_004155190.1 DUF4942 domain-containing protein -
KQH39_RS01235 (KQH39_01245) 246178..246426 + 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
KQH39_RS01240 (KQH39_01250) 246430..246705 + 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
KQH39_RS01245 (KQH39_01255) 247124..247324 + 201 Protein_239 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 223534..247472 23938
flank IS/Tn - - 247124..247348 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T205972 WP_004155189.1 NZ_CP076590:244815-245135 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T205972 NZ_CP101984:850018-850296 [Streptococcus mutans]
ATGCTAAAGATTAAGCAAACCAGACAATTTAAGAAATCCTTAAAAAAGGTAGTAAAACAAGGAAAAGATATTAACAAGCT
TTTTGCTATCGTAGAATTATTATGCCAAAAATCAGAACTTCCCTTAGCATTGAGAAATCATGAGTTGAAGGGAAGGTGGA
GAGGCATTAGAGAACTTCATATTGAATCAGACTGGCTGTTAGCCTATCAAGTTTTGGATGACGAACTTGTCTTACTCTTG
ATTGATACAGGTAGTCACGCACAGATGTTAGGAATGTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT205972 WP_004155188.1 NZ_CP076590:244455-244778 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT205972 NZ_CP101984:849759-850031 [Streptococcus mutans]
ATGGTTGTAAAAAATAAAGCAAATATCAATATCAAAATTGATTTGGAAGATAAAGCAGCAGCGGATGCTATTTTTGCTCA
CATGGGTCTAACAACAAGTGCTGCTGTTAATATGTTTATTAAACGTGTCATTGATGATCAAGCTCTGCCATTTACTCCAA
GAGTAAAAAATACTTTGGACATTGCTTTAGAGCAGGCTAAAAATGATGATGTTGAAACTTTTGATAGTTTTGATGATTGG
AAATCAGAGATGAGTGCCTATGCTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References