89363

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 3303142..3303669 Replicon chromosome
Accession NZ_CP024970
Organism Erwinia amylovora strain E-2

Toxin (Protein)


Gene name relE Uniprot ID D4HV72
Locus tag AD997_RS15450 Protein ID WP_004155192.1
Coordinates 3303142..3303417 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID D4HV71
Locus tag AD997_RS15455 Protein ID WP_004155191.1
Coordinates 3303421..3303669 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD997_RS15440 3298861..3302226 + 3366 WP_004155194.1 ATP-binding protein -
AD997_RS18190 3302375..3302527 - 153 WP_004163933.1 hypothetical protein -
AD997_RS15445 3302523..3302723 - 201 Protein_2874 transposase -
AD997_RS15450 3303142..3303417 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AD997_RS15455 3303421..3303669 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein Antitoxin
AD997_RS15460 3303766..3304599 - 834 WP_004155190.1 DUF4942 domain-containing protein -
AD997_RS15465 3304712..3305032 - 321 WP_004155189.1 TA system toxin CbtA family protein -
AD997_RS15470 3305069..3305392 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin -
AD997_RS15475 3305405..3305875 - 471 WP_004155187.1 DNA repair protein RadC -
AD997_RS15480 3305946..3306520 - 575 Protein_2881 DUF932 domain-containing protein -
AD997_RS15485 3306745..3306987 - 243 WP_004155184.1 hypothetical protein -
AD997_RS15495 3308197..3308394 + 198 WP_004155182.1 AlpA family phage regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3303142..3326289 23147
flank IS/Tn - - 3302499..3302723 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 11108.44 Da        Isoelectric Point: 5.5782

>T89363 WP_004155192.1 NZ_CP024970:c3303417-3303142 [Erwinia amylovora]
MEIQWTQKAQDDLERIYRFACQYSRQHADNVLERLLIGTSGLADHPRIGVSQIRYEPREVRKILFDDYDVHYEIQRDTIY
IVDLWHTREDR

Download         Length: 276 bp

>T89363 NZ_CP024970:c3303417-3303142 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9121.53 Da        Isoelectric Point: 8.7560

>AT89363 WP_004155191.1 NZ_CP024970:c3303669-3303421 [Erwinia amylovora]
MRQLAKQTISAQIPAELAAAVENLAIELDRSKSWVIKEALTAMIEERERRHQQILKGLADVDAGRVVSHADVVDFANKLK
KS

Download         Length: 249 bp

>AT89363 NZ_CP024970:c3303669-3303421 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EQZ9

Antitoxin

Source ID Structure
AlphaFold DB A0A831A0W7

References